College of Marine and Bio-Engineering, Yancheng Teachers University, Yancheng, Jiangsu 210095, PR China.
Eco-Environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, PR China.
Int J Syst Evol Microbiol. 2019 Nov;69(11):3332-3337. doi: 10.1099/ijsem.0.003131.
A beige-pigmented, Gram-stain-negative, aerobic, non-motile, rod-shaped bacterium, named 24, was isolated from sludge of a pesticide manufacturing factory in Nantong, Jiangsu Province, China. 16S rRNA gene sequence analysis revealed that strain 24 shared highest similarity with Ch07 (98.20 %), followed by SC-089 (98.07 %) and IMT-305 (98.03 %). Phylogenetic trees showed that strain 24 formed a distinct clade with IMT-305. The results of DNA-DNA hybridization tests showed that reassociation values were less than 45 % with respect to these closely related type strains. Strain 24 contained Q-8 and putrescine as the major respiratory quinone and polyamine, respectively. The main cellular fatty acids were summed feature 3 (Cω7c/Cω6), summed feature 2 (iso-C I/C 3-OH/C aldehyde), summed feature 8 (Cω7c/Cω6c) and C. The polar lipid profile included phosphatidylmethylethanolamin, phosphatidylethanolamine, phosphatidylglycerol, one unidentified phospholipid and one unidentified aminolipid. The G+C content was 56.83 mol%. Combined data from phenotypic, phylogenetic and DNA-DNA relatedness studies demonstrated that strain 24 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 24 (=CCTCC AB 2018057=KACC 19692).
从中国江苏省南通市一家农药制造厂的污泥中分离到一株呈浅棕色、革兰氏阴性、需氧、非运动、杆状的细菌，命名为 24 号。16S rRNA 基因序列分析显示，菌株 24 与 Ch07（98.20%）的相似度最高，其次是 SC-089（98.07%）和 IMT-305（98.03%）。系统发育树显示，菌株 24 与 IMT-305 形成了一个独特的分支。DNA-DNA 杂交试验的结果表明，与这些密切相关的模式菌株的再结合值小于 45%。菌株 24 含有 Q-8 和腐胺分别作为主要呼吸醌和聚胺。主要的细胞脂肪酸为总和特征 3（Cω7c/Cω6）、总和特征 2（iso-C I/C 3-OH/C 醛）、总和特征 8（Cω7c/Cω6c）和 C。极性脂谱包括磷脂酰甲硫氨酸乙醇胺、磷脂乙醇胺、磷脂酰甘油、一种未鉴定的磷脂和一种未鉴定的胺脂。G+C 含量为 56.83 mol%。表型、系统发育和 DNA-DNA 同源性研究的综合数据表明，菌株 24 代表了一个新的属种，建议将其命名为 sp. nov.。模式菌株为 24 号（=CCTCC AB 2018057=KACC 19692）。
