Ferreira Maria Onaira Gonçalves, Ribeiro Alessandra Braga, Rizzo Marcia S, de Jesus Oliveira Antonia Carla, Osajima Josy Anteveli, Estevinho Leticia M, Silva-Filho Edson C
Graduate Program in Materials Science, Campus Universitario Ministro Petrônio Portella, Federal University of Piaui, Teresina 64049-550, PI, Brazil.
CBQF-Centre of Biotechnology and Fine Chemistry-Associate Laboratory, Faculty of Biotechnology, Catholic University of Portugal, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal.
Biomedicines. 2022 Apr 14;10(4):899. doi: 10.3390/biomedicines10040899.
Wounds are considered a clinically critical issue, and effective treatment will decrease complications, prevent chronic wound formation, and allow rapid healing. The development of products based on naturally occurring materials is an efficient approach to wound healing. Natural polysaccharides can mimic the extracellular matrix and promote cell growth, thus making them attractive for wound healing. In this context, the aim of this work was to produce a gel based on chicha gum, chitosan, and oil (CGCHO) for wound treatment. TG and DTG analyzed the thermal behavior of the materials, and SEM investigated the surface roughness. The percentages of total phenolic compounds, flavonoids, and antioxidants were determined, presenting a value of 81.811 ± 7.257 µmol gallic acid/g oil, 57.915 ± 0.305 µmol quercetin/g oil, and 0.379 mg/mL, respectively. The anti-inflammatory was determined, presenting a value of 10.35 ± 1.46% chicha gum, 16.86 ± 1.00% oil, 10.17 ± 1.05% CGCHO, and 15.53 ± 0.65% chitosan, respectively. The materials were tested against Gram-negative () and Gram-positive () bacteria and a fungus (). The CGCHO formulation showed better antimicrobial activity against Gram-positive bacteria. In addition, an in vivo wound healing study was also performed. After 21 days of treatment, the epidermal re-epithelialization process was observed. CGCHO showed good thermal stability and roughness that can help in cell growth and promote the tissue healing process. In addition to the good results observed for the antimicrobial, antioxidant, anti-inflammatory activities and providing wound healing, they provided the necessary support for the healing process, thus representing a new approach to the wound healing process.
伤口被认为是一个临床关键问题，有效的治疗将减少并发症、预防慢性伤口形成并促进快速愈合。基于天然材料开发产品是促进伤口愈合的有效途径。天然多糖可以模拟细胞外基质并促进细胞生长，因此使其成为伤口愈合的理想材料。在此背景下，本研究旨在制备一种基于奇恰胶、壳聚糖和油（CGCHO）的凝胶用于伤口治疗。热重分析（TG）和微商热重分析（DTG）研究了材料的热行为，扫描电子显微镜（SEM）研究了表面粗糙度。测定了总酚类化合物、黄酮类化合物和抗氧化剂的含量，分别为81.811±7.257微摩尔没食子酸/克油、57.915±0.305微摩尔槲皮素/克油和0.379毫克/毫升。测定了抗炎活性，奇恰胶、油、CGCHO和壳聚糖的抗炎率分别为10.35±1.46%、16.86±1.00%、10.17±1.05%和15.53±0.65%。对材料进行了抗革兰氏阴性菌、革兰氏阳性菌和真菌的测试。CGCHO配方对革兰氏阳性菌表现出更好的抗菌活性。此外，还进行了体内伤口愈合研究。治疗21天后，观察到表皮再上皮化过程。CGCHO表现出良好的热稳定性和粗糙度，有助于细胞生长并促进组织愈合过程。除了在抗菌、抗氧化、抗炎活性以及促进伤口愈合方面取得的良好结果外，它们还为愈合过程提供了必要的支持，从而代表了一种伤口愈合的新方法。
