Department of Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan (DUVASU), Mathura, India.
Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan (DUVASU), Mathura, India.
J Tissue Viability. 2021 Feb;30(1):59-66. doi: 10.1016/j.jtv.2020.12.002. Epub 2020 Dec 17.
Cell-based therapy has emerged as promising strategy for chronic and impaired wounds treatment. Current research is focused on developing biomaterial systems that act as a niche for mesenchymal stem cells (MSCs) to promote wound healing through paracrine molecular cascading. This study was aimed to evaluate the wound healing potential of Velgraft, a ready-to-use biodegradable artificial skin substitute, on excision wound in goats.
Twelve male goats were randomized divided in to three groups of four animals each. After infliction of surgical wound, Velgraft and Soframycin were applied on wounds of the animals of Groups II and III while Group I (sham operated) served as control. Wound diameters were measured at pre-defined time-points for determination of progressive wound healing up to 28 days. Skin sections were stained using Hematoxylin and eosin (H&E) for examining the histoarchitectural changes, Masson trichome staining for ascertaining collagen synthesis and immunohistochemistry for expression of CD31, VEGF and TGF-β1 proteins to determine post-treatment angiogenesis in the inflicted wounds.
Velgraft application appreciably enhanced wound closure by day 21 which was confirmed through restoration of the normal skin architecture as evident based on histopathological examination and characterized by complete regeneration of epidermal layers, collagen fibers, blood capillaries and hair follicular formation. Stimulation of angiogenesis markers was also observed at different time-points post-Velgraft application; which is suggestive of the improved angiogenesis and vasculogenesis.
Velgraft facilitates wound healing by augmenting early wound closure, enhancing collagen synthesis and deposition, trichosis development and promoting revascularization and epidermal layers restoration.
细胞疗法已成为治疗慢性和受损伤口的有前途的策略。目前的研究重点是开发生物材料系统，作为间充质干细胞（MSCs）的龛位，通过旁分泌分子级联促进伤口愈合。本研究旨在评估 Velgraft（一种即用型可生物降解的人工皮肤替代物）在山羊切除伤中的伤口愈合潜力。
12 只雄性山羊随机分为三组，每组 4 只。在造成手术伤口后，将 Velgraft 和 Soframycin 应用于第 II 组和第 III 组动物的伤口，而第 I 组（假手术）作为对照。在预定的时间点测量伤口直径，以确定直至 28 天的伤口愈合进展。使用苏木精和伊红（H&E）染色皮肤切片，检查组织形态学变化，Masson 三色染色确定胶原蛋白合成，免疫组织化学检测 CD31、VEGF 和 TGF-β1 蛋白的表达，以确定受伤部位的治疗后血管生成。
Velgraft 的应用在第 21 天显著促进了伤口闭合，这通过组织病理学检查证实了正常皮肤结构的恢复，其特征是表皮层、胶原纤维、毛细血管和毛囊的完全再生。在 Velgraft 应用后的不同时间点也观察到了血管生成标志物的刺激，这表明血管生成和血管发生得到了改善。
Velgraft 通过增加早期伤口闭合、增强胶原蛋白合成和沉积、毛发生长以及促进再血管化和表皮层恢复来促进伤口愈合。
