Maoga Jane B, Riaz Muhammad A, Mwaura Agnes N, Scheiner-Bobis Georgios, Mecha Ezekiel, Omwandho Charles O A, Meinhold-Heerlein Ivo, Konrad Lutz
Department of Gynecology and Obstetrics, Justus Liebig University, 35392 Giessen, Germany.
Institute of Veterinary-Physiology and Biochemistry, Justus Liebig University, 35392 Giessen, Germany.
Diagnostics (Basel). 2022 Mar 22;12(4):779. doi: 10.3390/diagnostics12040779.
Matrix metalloproteinases (MMPs) play an important role in menstruation and endometriosis; however, the membrane-type matrix metalloproteinases (MT-MMPs) are not well studied in endometriosis and adenomyosis. We analyzed MT2-MMP (MMP15) and MT3-MMP (MMP16) in eutopic endometrium with and without endometriosis and with and without adenomyosis and ectopic endometrium of deep infiltrating endometriosis (DIE), peritoneal endometriosis (PE), and ovarian endometriosis (Ov) by immunohistochemistry. Preferential expression of both proteins was observed in the glandular and luminal epithelial cells of the eutopic endometrium of patients with and without endometriosis with a ~2.5-fold stronger expression of MT3-MMP compared to MT2-MMP. We did not observe any differences during menstrual cycling and in eutopic endometrium of patients with and without endometriosis. Similarly, eutopic endometrium and adenomyotic tissue with and without endometriosis showed similar protein levels of MT2-MMP and MT3-MMP. In contrast, MT2-MMP and MT3-MMP protein was decreased in ectopic compared to eutopic endometrium and adenomyosis. The similar expression of MT2-MMP and MT3-MMP in eutopic endometrium in patients with and without endometriosis in contrast to the impaired expression in ectopic endometrium suggests that alterations occur after and not before endometrial implantation possibly by distinct interactions with the different environments. The differential protein expression of MT2/3-MMP in adenomyosis compared to endometriosis might suggest a different pathogenesis pathway for the two diseases.
基质金属蛋白酶（MMPs）在月经和子宫内膜异位症中起重要作用；然而，膜型基质金属蛋白酶（MT-MMPs）在子宫内膜异位症和子宫腺肌病中的研究尚不充分。我们通过免疫组织化学分析了MT2-MMP（MMP15）和MT3-MMP（MMP16）在有或无子宫内膜异位症、有或无子宫腺肌病的在位子宫内膜以及深部浸润性子宫内膜异位症（DIE）、腹膜子宫内膜异位症（PE）和卵巢子宫内膜异位症（Ov）的异位子宫内膜中的表达情况。在有或无子宫内膜异位症患者的在位子宫内膜的腺上皮和腔上皮细胞中均观察到这两种蛋白的优先表达，与MT2-MMP相比，MT3-MMP的表达强度约强2.5倍。我们在月经周期中以及有或无子宫内膜异位症患者的在位子宫内膜中均未观察到任何差异。同样，有或无子宫内膜异位症的在位子宫内膜和子宫腺肌病组织中MT2-MMP和MT3-MMP的蛋白水平相似。相比之下，与在位子宫内膜和子宫腺肌病相比，异位内膜中MT2-MMP和MT3-MMP蛋白减少。有或无子宫内膜异位症患者的在位子宫内膜中MT2-MMP和MT3-MMP表达相似，而异位内膜中表达受损，这表明改变发生在子宫内膜植入之后而非之前，可能是由于与不同环境的独特相互作用所致。与子宫内膜异位症相比，子宫腺肌病中MT2/3-MMP的蛋白表达差异可能提示这两种疾病的发病机制不同。
