Radulski Łukasz, Krajewska-Wędzina Monika, Lipiec Marek, Szulowski Krzysztof
National Veterinary Research Institute, 24-100 Puławy, Poland.
Animals (Basel). 2022 Apr 8;12(8):964. doi: 10.3390/ani12080964.
The most numerous group of bacteria in the genus is the nontuberculous mycobacteria. Currently, over 200 species of bacteria have been classified as belonging to this group, of which approximately 30 are pathogenic to humans and animals. complex numbers among these pathogenic species. The submandibular lymph nodes of a wild boar shot by a hunter were examined in order to confirm or exclude infection with bacteria of the genus . In culture, a bacterial isolate was obtained after 12 days of incubation on Petragnani and Stonebrink media. A multiplex PCR clearly indicated that the isolate was a nontuberculous mycobacterium. The results of species identification attempts via both molecular biology methods and mass spectrometry confirmed that the isolated strain belonged to MKC. The described case of a wild boar infection with MKC is the first documented case in Poland and only the second in Europe, and in confirming the presence of this pathogen among free-living animals, this report implies that MKC is of great concern. Our research elucidates some specifics of wild boar mycobacteriosis and may be used to instill awareness in the public of the dangers of dressing hunt prey or consuming its meat in ignorance of safe procedures, which can contribute to the transmission of the pathogen to humans.
该属中数量最多的细菌群是非结核分枝杆菌。目前，已有200多种细菌被归类为该菌群，其中约30种对人类和动物具有致病性。这些致病物种中的 复合体数量众多。为了确认或排除感染该属细菌，对一名猎人射杀的野猪的下颌下淋巴结进行了检查。在培养过程中，在佩特拉尼亚尼培养基和斯通布林克培养基上孵育12天后获得了一种细菌分离株。多重聚合酶链反应清楚地表明该分离株是非结核分枝杆菌。通过分子生物学方法和质谱法进行物种鉴定的结果证实，分离出的菌株属于MKC。所描述的野猪感染MKC的病例是波兰有记录的首例，也是欧洲的第二例，并且在确认这种病原体在自由生活的动物中的存在时，本报告意味着MKC令人高度关注。我们的研究阐明了野猪分枝杆菌病的一些细节，并可用于让公众意识到在不知道安全程序的情况下处理狩猎猎物或食用其肉的危险，这可能会导致病原体传播给人类。
