Damina Sadiq Mohammed, Barnes David Atomanyi, Inuwa Bitrus, Ularamu Gulak Hussaini, Bello Mohammed, Okaiyeto Olu Solomon, Kudi Ayuba Caleb, Thapa Jeewan, Nakajima Chie, Suzuki Yasuhiko
National Veterinary Research Institute, P.M.B.01, Vom 930103, Nigeria.
Division of Bioresources, Hokkaido University International Institute for Zoonosis Control, Kita 20 Nishi 10, Kita-ku, Hokkaido 001-0020, Japan.
Curr Issues Mol Biol. 2023 Jul 19;45(7):6055-6066. doi: 10.3390/cimb45070382.
Bovine tuberculosis is endemic in Nigeria with control measures as provided by the laws of the country being minimally enforced mostly at the abattoirs only. This study focused on bovine tuberculosis in Adamawa and Gombe States. Tuberculosis lesions were observed in 183 of 13,688 slaughtered cattle in the regions between June and December 2020. Analysis of tissue samples resulted in 17 isolates, predominantly from Gombe State. Spoligotyping identified four spoligotypes, including SB0944, SB1025, SB1104, and one novel pattern. MIRU-VNTR analysis further differentiated these spoligotypes into eight profiles. All isolates belonged to the Af1 clonal complex. The study emphasises the need for broader coverage and more isolates to comprehensively understand the molecular epidemiology of bovine tuberculosis in Nigeria. To enhance research and surveillance, a cost-effective approach is proposed, utilising a discriminatory VNTR panel comprising five or nine loci. The five-locus panel consists of ETR-C, QUB26, QUB11b, MIRU04, and QUB323. Alternatively, the nine-locus panel includes ETR-A, ETR-B, QUB11a, and MIRU26. Implementing this approach would provide valuable insights into the genetic diversity of strains in Nigeria. These findings are crucial for developing effective control measures and minimising the impact of bovine tuberculosis on both animal and human health.
牛结核病在尼日利亚呈地方流行，该国法律规定的控制措施大多仅在屠宰场得到最低限度的执行。本研究聚焦于阿达马瓦州和贡贝州的牛结核病。2020年6月至12月期间，在该地区13688头屠宰牛中，有183头观察到结核病病变。对组织样本的分析产生了17株分离株，主要来自贡贝州。间隔寡核苷酸分型鉴定出四种间隔寡核苷酸分型，包括SB0944、SB1025、SB1104以及一种新的模式。多位点可变数目串联重复序列分析进一步将这些间隔寡核苷酸分型分为八个谱系。所有分离株均属于Af1克隆复合体。该研究强调需要更广泛的覆盖范围和更多的分离株，以全面了解尼日利亚牛结核病的分子流行病学。为加强研究和监测，提出了一种经济有效的方法，即使用由五个或九个位点组成的具有鉴别力的可变数目串联重复序列组合。五位点组合由ETR-C、QUB26、QUB11b、MIRU04和QUB323组成。或者，九位点组合包括ETR-A、ETR-B、QUB11a和MIRU-26。实施这种方法将为尼日利亚菌株的遗传多样性提供有价值的见解。这些发现对于制定有效的控制措施以及将牛结核病对动物和人类健康的影响降至最低至关重要。
