Nishi K, Tokunaga A, Shimizu Y, Yoshiyuki T, Wada M, Matsukura N, Tanaka N, Onda M, Asano G
Cancer. 1987 Apr 1;59(7):1328-32. doi: 10.1002/1097-0142(19870401)59:7<1328::aid-cncr2820590716>3.0.co;2-c.
Tissues from primary human gastric cancers were examined for intracellular estradiol (E2) by using the avidin-biotin-peroxidase complex (ABC) immunohistochemical method on formalin-fixed paraffin-embedded sections. Reaction products of E2 were located only in the cytoplasm of the cancer cells, and not detected in noncancerous gastric epithelium. E2-positive tissues were found in 23 (44.2%) of 52 male patients, seven (20.6%) of 34 female patients and a total of 30 (34.9%) of 86 patients. In male patients, E2-positive cases occurred without age distinction. In female patients, however, E2 was not found in patients in older age groups, especially patients in the postmenopausal state. Microscopically, E2 was found frequently in intestinal type of cancers in male patients and in cancer with scirrhous growth pattern, in female patients. This is the first report of the demonstration of E2 in gastric cancer. The findings suggest that hormonal factors are involved in gastric cancer, and that the cancers contain endocrinic characteristics.
采用抗生物素蛋白-生物素-过氧化物酶复合物（ABC）免疫组织化学方法，对取自原发性人类胃癌的组织进行检测，以确定细胞内雌二醇（E2）水平。检测在经福尔马林固定、石蜡包埋的切片上进行。E2的反应产物仅位于癌细胞的细胞质中，在非癌性胃上皮中未检测到。52例男性患者中有23例（44.2%）、34例女性患者中有7例（20.6%）检测到E2阳性组织，86例患者中共有30例（34.9%）检测到E2阳性。在男性患者中，E2阳性病例无年龄差异。然而，在女性患者中，老年组患者，尤其是绝经后患者未检测到E2。显微镜下观察发现，男性患者的肠型癌以及女性患者的硬癌生长模式的癌症中，E2的出现频率较高。这是关于在胃癌中检测到E2的首次报告。这些发现表明，激素因素与胃癌有关，且这些癌症具有内分泌特征。
