Allouche Rania, Hafeez Zeeshan, Papier Florent, Dary-Mourot Annie, Genay Magali, Miclo Laurent
CALBINOTOX, Université de Lorraine, F-54000 Nancy, France.
Foods. 2022 Apr 16;11(8):1157. doi: 10.3390/foods11081157.
, a lactic acid bacterium widely used in the dairy industry, is consumed regularly by a significant proportion of the population. Some strains show in vitro anti-inflammatory activity which is not fully understood. We hypothesized that peptides released from the surface proteins of this bacterium during digestion could be implied in this activity. Consequently, we prepared a peptide hydrolysate by shaving and hydrolysis of surface proteins using trypsin, and the origin of peptides was checked by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Most of the identified peptides originated from bacterial cell surface proteins. The anti-inflammatory activity of peptide hydrolysate was investigated under inflammatory conditions in two cell models. Peptide hydrolysate significantly decreased secretion of pro-inflammatory cytokine IL-8 in lipopolysaccharide (LPS)-stimulated human colon epithelial HT-29 cells. It also reduced the production of pro-inflammatory cytokines IL-8, IL-1β and the protein expression levels of Pro-IL-1β and COX-2 in LPS-stimulated THP-1 macrophages. The results showed that peptides released from bacterial surface proteins by a pancreatic protease could therefore participate in an anti-inflammatory activity of LMD-9 and could prevent low-grade inflammation.
乳酸乳球菌是一种广泛应用于乳制品行业的乳酸菌，相当一部分人经常食用。一些菌株表现出体外抗炎活性，但其机制尚未完全明确。我们推测，该细菌在消化过程中从表面蛋白释放的肽可能参与了这一活性。因此，我们通过胰蛋白酶刮擦和水解表面蛋白制备了肽水解产物，并通过液相色谱-串联质谱（LC-MS/MS）分析检查了肽的来源。大多数鉴定出的肽源自细菌细胞表面蛋白。在两种细胞模型的炎症条件下研究了肽水解产物的抗炎活性。肽水解产物显著降低了脂多糖（LPS）刺激的人结肠上皮HT-29细胞中促炎细胞因子IL-8的分泌。它还降低了LPS刺激的THP-1巨噬细胞中促炎细胞因子IL-8、IL-1β的产生以及Pro-IL-1β和COX-2的蛋白表达水平。结果表明，胰蛋白酶从细菌表面蛋白释放的肽因此可以参与乳酸乳球菌LMD-9的抗炎活性，并可以预防低度炎症。
