Su Ruey-Chih, Leu Jyh-Gang, Chen Yuan-Hsin, Chen Chao-Yi, Yang Yi-Feng, Yen Chih-Cheng, Chou Shiu-Huey, Liang Yao-Jen
Department of Life Science, Fu-Jen Catholic University, New Taipei City 242062, Taiwan.
Graduate Institute of Applied Science and Engineering, Fu-Jen Catholic University, New Taipei City 242062, Taiwan.
Life (Basel). 2022 Mar 30;12(4):507. doi: 10.3390/life12040507.
The number of diagnosed diabetic patients is increasing worldwide. Many people with diabetes develop wounds that are slow to, or never, heal, which can lead to serious health issues. Diabetes causes long-term excessive blood glucose buildup in human body, which leads to an over-reactive inflammatory response and excessive oxidative stress. As a result, varied wound healing effects were observed according to different circumstances and stage of healing. We used two diabetic wound animal models to analyze the wound healing effect of ointment in either topical application and/or oral administration, and explored its mechanism by Western blot analysis. The results showed that topical treatment can significantly promote wound healing. The increased expressions of angiopoietin 1 and angiopoietin 2 protein and reduction of CD68 expression were found around wound area. Simultaneous treatment of oral and topical ointment did not show an accelerated healing effect in our animal model. This study is the first report to demonstrate the effect of topical application of ointment on diabetic wounds healing, and its relationship with angiogenesis. This may also open a new field for future development and application of Taiwan .
全球范围内，被诊断出的糖尿病患者数量正在增加。许多糖尿病患者会出现伤口愈合缓慢或根本无法愈合的情况，这可能导致严重的健康问题。糖尿病会导致人体长期血糖过高，进而引发过度的炎症反应和氧化应激。因此，根据不同的情况和愈合阶段，会观察到不同的伤口愈合效果。我们使用两种糖尿病伤口动物模型来分析药膏局部应用和/或口服给药时的伤口愈合效果，并通过蛋白质印迹分析探索其作用机制。结果表明，局部治疗可显著促进伤口愈合。在伤口周围区域发现血管生成素1和血管生成素2蛋白表达增加，而CD68表达减少。在我们的动物模型中，口服和局部同时使用药膏并未显示出加速愈合的效果。本研究首次报道了局部应用药膏对糖尿病伤口愈合的作用及其与血管生成的关系。这也可能为药膏未来的开发和应用开辟一个新领域。
