Soontarach Rosesathorn, Srimanote Potjanee, Enright Mark C, Blundell-Hunter George, Dorman Matthew J, Thomson Nicholas R, Taylor Peter W, Voravuthikunchai Supayang P
Division of Biological Science, Faculty of Science and Natural Product Research Center of Excellence, Prince of Songkla University, Songkhla 90110, Thailand.
Center of Antimicrobial Biomaterial Innovation-Southeast Asia, Prince of Songkla University, Songkhla 90110, Thailand.
Pharmaceuticals (Basel). 2022 Apr 2;15(4):443. doi: 10.3390/ph15040443.
Nineteen bacteriophages against five main capsular types of multidrug-resistant were isolated from tertiary care hospital sewage. Eight representative phages from each capsular type were characterized and tested for their biological properties. The biological features revealed that phages T1245, T444, and T515 had a large burst size of more than 420 pfu/mL, together with a short latent period lasting less than 6 min, and were readily adsorbed to a bacterial host within 10 min. Moreover, these phages demonstrated host specificity and stability over a broad range of temperatures (-20 to 60 °C) and pH (5.0-9.0). A whole-genome analysis of six lytic and two temperate phages revealed high genomic similarity with double-stranded DNA between 40 and 50 kb and G + C content of 38-39%. The protein compositions disclosed the absence of toxin-coding genes. The phylogenic results, together with morphological micrographs, confirmed that three selected phages (T1245, T444, and T515) belong to the Podoviridae family within the order Caudovirales. The biological data and bioinformatics analysis indicated that these novel phages possess important enzymes, including depolymerase and endolysin, which could be further developed as promising alternative antibacterial agents to control infections.
从三级医院污水中分离出19种针对5种主要耐药荚膜类型的噬菌体。对每种荚膜类型的8种代表性噬菌体进行了特性鉴定并测试了其生物学特性。生物学特性表明，噬菌体T1245、T444和T515具有大于420 pfu/mL的大裂解量，潜伏期短，持续时间不到6分钟，并且在10分钟内易于吸附到细菌宿主上。此外，这些噬菌体在广泛的温度（-20至60°C）和pH（5.0 - 9.0）范围内表现出宿主特异性和稳定性。对6种裂解性噬菌体和2种温和性噬菌体的全基因组分析显示，其基因组与40至50 kb的双链DNA高度相似，G + C含量为38 - 39%。蛋白质组成表明不存在毒素编码基因。系统发育结果以及形态显微照片证实，三种选定的噬菌体（T1245、T444和T515）属于长尾噬菌体目下的短尾噬菌体科。生物学数据和生物信息学分析表明，这些新型噬菌体拥有重要的酶，包括解聚酶和内溶素，可进一步开发成为控制感染的有前景的替代抗菌剂。
