Sánchez Francisco S Lozano, Martínez José A Carnicero, Méndez-García Lucía, García-Cenador M Begoña, Pericacho Miguel
Service of Angiology, Vascular and Endovascular Surgery, University Hospital of Salamanca (HUS), 37007 Salamanca, Spain.
Department of Surgery, University of Salamanca (USAL), 37008 Salamanca, Spain.
J Pers Med. 2022 Mar 25;12(4):528. doi: 10.3390/jpm12040528.
Surgery on varicose veins (crossectomy and stripping) may lead to recurrence, with clinical and socioeconomic repercussions. The etiopathogenesis of varicose veins has yet to be fully understood.
Study the expression of endoglin and other molecules involved in the neovascularisation process in patients suffering from this disease.
Total of 43 patients that have undergone surgery for varicose veins (24 primary and 19 recurrent). Endoglin and other molecules were identified on the venous wall (proximal -saphenofemoral junction- and distal), via real-time RT-PCR, and in serum, via ELISA: endoglin (Eng), vascular endothelial growth factor (VEGF-A), its receptors 1 and 2 (VEGFR1 or FLT1), (VEGFR2 or FLK), and the hypoxia-inducible factor (HIF-1A). All the patients signed a consent form.
The recurrent group recorded a higher expression of Eng, VEGF-A, VEGFR1, and VEGFR2 at the level of proximal venous wall compared to the primary group. HIF-1A did not record any differences. As regards the determination of the distal venous wall, no markers recorded differences between the groups. Among the serum determinations, only sFLT1 recorded a significant drop among the patients with recurrent varicose veins.
Patients with recurrent varicose veins record a higher expression of endoglin and other markers of angiogenesis in proximal veins. Endoglin in the blood (sEng) serves no apparent purpose in recurrent varicose veins.
静脉曲张手术（横断术和剥脱术）可能导致复发，产生临床和社会经济影响。静脉曲张的病因发病机制尚未完全明确。
研究患有这种疾病的患者中内皮糖蛋白及其他参与新生血管形成过程的分子的表达情况。
共有43例接受了静脉曲张手术的患者（24例初次发病患者和19例复发患者）。通过实时逆转录聚合酶链反应在静脉壁（近端 - 大隐静脉股静脉交界处 - 和远端）鉴定内皮糖蛋白及其他分子，并通过酶联免疫吸附测定法在血清中鉴定：内皮糖蛋白（Eng）、血管内皮生长因子（VEGF - A）、其受体1和2（VEGFR1或FLT1）、（VEGFR2或FLK）以及缺氧诱导因子（HIF - 1A）。所有患者均签署了知情同意书。
与初次发病组相比，复发组在近端静脉壁水平记录到Eng、VEGF - A、VEGFR1和VEGFR2的表达较高。HIF - 1A未记录到任何差异。关于远端静脉壁的测定，两组之间没有标志物记录到差异。在血清测定中，只有可溶性FLT1在复发性静脉曲张患者中记录到显著下降。
复发性静脉曲张患者在近端静脉中记录到内皮糖蛋白和其他血管生成标志物的较高表达。血液中的内皮糖蛋白（sEng）在复发性静脉曲张中似乎没有明显作用。
