Persson K M, Forsgren A
Diagn Microbiol Infect Dis. 1987 Feb;6(2):175-7. doi: 10.1016/0732-8893(87)90104-0.
Attempts were made to isolate group B streptococci (GBS) from 382 urethral and 140 rectal specimens by overnight incubation in three variants of Todd Hewitt broth, followed by subculture on human blood agar plates. All three broths prepared contained 5% sheep blood, but one contained no antibiotics (THB), while the other two contained gentamicin and nalidixic acid at concentrations of 0.8 microgram/ml and 1.5 micrograms/ml (THBL), and 8 micrograms/ml and 15 micrograms/ml (THBH), respectively. THBL gave the highest isolation frequency both for urethral and rectal specimens. While THBH was superior to THB for isolating GBS in rectal specimens, for urethral samples they were equally effective. Direct plating of 154 specimens on blood agar was less effective than was enrichment in any of the three broths followed by plating on blood agar.
通过在三种不同变体的托德-休伊特肉汤中过夜培养，随后在人血琼脂平板上进行传代培养，尝试从382份尿道标本和140份直肠标本中分离B族链球菌（GBS）。所制备的三种肉汤均含有5%的羊血，但一种不含抗生素（THB），而另外两种分别含有浓度为0.8微克/毫升和1.5微克/毫升的庆大霉素和萘啶酸（THBL），以及8微克/毫升和15微克/毫升（THBH）。THBL对尿道和直肠标本的分离频率最高。虽然THBH在分离直肠标本中的GBS方面优于THB，但对于尿道标本，它们的效果相同。将154份标本直接接种在血琼脂平板上的效果不如在三种肉汤中的任何一种中进行增菌培养后再接种在血琼脂平板上。
