Duan Haiyan, Fu Cong, Du Guilin, Xie Shiqiu, Liu Min, Zhang Baoguo, Shi Jiping, Sun Junsong
Laboratory of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Shanghai 201210, China.
School of Life Science and Technology, Shanghai Tech University, Shanghai 201210, China.
Microorganisms. 2022 Mar 25;10(4):709. doi: 10.3390/microorganisms10040709.
The effects of Lysinibacillus sp. LF-N1 and Penicillium oxalicum DH-1 inoculants (LFPO group) on compost succession and the microbial dynamic structure of co-composting wheat straw and cow manure composting were investigated. The inoculants contributed to longer thermophilic stages, higher temperatures (62.8 °C) and lower microbial diversity in the LFPO treatment compared to the control group (CK). Moreover, LFPO inoculation increased the germination index and accelerated organic matter and lignocellulose degradation in the compost. Microbial analysis confirmed that the inoculants effectively altered the microbial communities. The predominant biomarkers for bacteria and fungi in inoculated compost were members of Lysinibacillus and Penicillium, respectively. Functional prediction showed greater lignocellulose degradation and less pathogen accumulation in the LFPO group. The cooccurrence network analysis showed that the network structure in LFPO compost was greatly simplified compared to that in CK. Bacterial cluster A was dominated by Lysinibacillus, and fungal cluster B was represented by Penicillium, which were significantly correlated with temperature and lignocellulose degradation, respectively (p < 0.05). These results demonstrated that the LF-N1 and DH-1 inoculants drove the bacterial and fungal assemblies to induce physicochemical property changes during cocomposting.
研究了赖氨酸芽孢杆菌LF-N1和草酸青霉DH-1接种剂（LFPO组）对小麦秸秆和牛粪混合堆肥过程中堆肥演替及微生物动态结构的影响。与对照组（CK）相比，接种剂使LFPO处理的嗜热阶段更长、温度更高（62.8℃）且微生物多样性更低。此外，接种LFPO提高了堆肥的发芽指数，加速了堆肥中有机物和木质纤维素的降解。微生物分析证实，接种剂有效地改变了微生物群落。接种堆肥中细菌和真菌的主要生物标志物分别是赖氨酸芽孢杆菌属和青霉属的成员。功能预测表明，LFPO组的木质纤维素降解能力更强，病原体积累更少。共现网络分析表明，与CK相比，LFPO堆肥中的网络结构大大简化。细菌簇A以赖氨酸芽孢杆菌为主，真菌簇B以青霉为主，它们分别与温度和木质纤维素降解显著相关（p<0.05）。这些结果表明，LF-N1和DH-1接种剂推动细菌和真菌群落组装，在混合堆肥过程中引起理化性质变化。
