Koch Maximilian, Palarie Victor, Koch Lisa, Burkovski Andreas, Zulla Manuel, Rosiwal Stefan, Karl Matthias
Division of Microbiology, Department of Biology, Friedrich-Alexander-Universität Erlangen-Nürnberg, 91058 Erlangen, Germany.
Laboratory of Tissue Engineering and Cellular Cultures, State University of Medicine and Pharmacy "N. Testemitanu", 2004 Chisinau, Moldova.
Microorganisms. 2022 Apr 7;10(4):782. doi: 10.3390/microorganisms10040782.
While numerous approaches have meanwhile been described, sufficient disinfection of root canals is still challenging, mostly due to limited access and the porous structure of dentin. Instead of using different rinsing solutions and activated irrigation, the electrolysis of saline using boron-doped diamond (BDD) electrodes thereby producing reactive oxygen species may be an alternative approach. In a first step, experiments using extracted human teeth incubated with multispecies bacterial biofilm were conducted. The charge quantities required for electrochemical disinfection of root canals were determined, which were subsequently applied in an animal trial using an intraoral canine model. It could be shown that also under realistic clinical conditions, predictable disinfection of root canals could be achieved using BDD electrodes. The parameters required are in the range of 5.5 to 7.0 V and 9 to 38 mA, applied for 2.5 to 6.0 min with approximately 5 to 8 mL of saline. The direct generation of disinfective agents inside the root canal seems to be advantageous especially in situations with compromised access and limited canal sizes. The biologic effect with respect to the host reaction on BDD-mediated disinfection is yet to be examined.
尽管与此同时已经描述了许多方法，但根管的充分消毒仍然具有挑战性，主要是由于进入受限和牙本质的多孔结构。使用掺硼金刚石（BDD）电极电解盐水从而产生活性氧物种，而不是使用不同的冲洗溶液和活化冲洗，可能是一种替代方法。第一步，进行了使用与多物种细菌生物膜一起孵育的拔除人类牙齿的实验。确定了根管电化学消毒所需的电荷量，随后将其应用于使用口腔内犬模型的动物试验中。结果表明，即使在实际临床条件下，使用BDD电极也可以实现可预测的根管消毒。所需参数为5.5至7.0V和9至38mA，使用约5至8mL盐水施加2.5至6.0分钟。根管内消毒剂的直接产生似乎特别有利于进入受限和根管尺寸有限的情况。关于BDD介导的消毒对宿主反应的生物学效应尚待研究。
