Koch Maximilian, Burkovski Andreas, Zulla Manuel, Rosiwal Stefan, Geißdörfer Walter, Dittmar Roman, Grobecker-Karl Tanja
Microbiology Division, Department of Biology, University of Erlangen-Nuremberg, 91058 Erlangen, Germany.
Division of Ultra-Hard Coatings, Department of Material Sciences, University of Erlangen-Nuremberg, 91058 Erlangen, Germany.
J Clin Med. 2020 Sep 21;9(9):3036. doi: 10.3390/jcm9093036.
No proper treatment option for peri-implantitis exists yet. Based on previous studies showing the in vitro effectiveness of electrochemical disinfection using boron-doped diamond electrodes, novel double diamond electrodes (DDE) were tested here. Using a ceramic carrier and a laser structuring process, a clinically applicable electrode array was manufactured. Roughened metal discs ( = 24) made from Ti-Zr alloy were exposed to the oral cavities of six volunteers for 24 h in order to generate biofilm. Then, biofilm removal was carried out either using plastic curettes and chlorhexidine digluconate or electrochemical disinfection. In addition, dental implants were contaminated with ex vivo multispecies biofilm and disinfected using DDE treatment. Bacterial growth and the formation of biofilm polymer were determined as outcome measures. Chemo-mechanical treatment could not eliminate bacteria from roughened surfaces, while in most cases, a massive reduction of bacteria and biofilm polymer was observed following DDE treatment. Electrochemical disinfection was charge- and time-dependent and could also not reach complete disinfection in all instances. Implant threads had no negative effect on DDE treatment. Bacteria exhibit varying resistance to electrochemical disinfection with , sp., , , and surviving 5 min of DDE application at 6 V. Electrochemical disinfection is promising but requires further optimization with respect to charge quantity and application time in order to achieve disinfection without harming host tissue.
目前尚无针对种植体周围炎的合适治疗方案。基于先前研究显示掺硼金刚石电极电化学消毒在体外的有效性，本文对新型双金刚石电极（DDE）进行了测试。利用陶瓷载体和激光结构化工艺，制造出了一种临床适用的电极阵列。由钛锆合金制成的粗糙金属圆盘（ = 24）被置于6名志愿者口腔中24小时以形成生物膜。然后，使用塑料刮匙和葡萄糖酸氯己定或进行电化学消毒来去除生物膜。此外，将牙种植体用体外多菌种生物膜污染，并使用DDE处理进行消毒。将细菌生长和生物膜聚合物的形成作为观察指标。化学机械处理无法从粗糙表面清除细菌，而在大多数情况下，DDE处理后观察到细菌和生物膜聚合物大量减少。电化学消毒与电荷和时间有关，且在所有情况下也无法达到完全消毒。种植体螺纹对DDE处理没有负面影响。细菌对6 V电压下DDE处理5分钟的电化学消毒表现出不同的抗性，其中 、 种、 、 、 存活下来。电化学消毒前景广阔，但需要在电荷量和应用时间方面进一步优化，以实现消毒而不损害宿主组织。
