Binsalah Mohammed, Devanesan Sandhanasamy, AlSalhi Mohamad S, Nooh Abdullrahman, Alghamdi Osama, Nooh Nasser
Department of Oral and Maxillofacial Surgery, College of Dentistry, King Saud University, Riyadh 11545, Saudi Arabia.
Research Chair in Laser Diagnosis of Cancers, Department of Physics and Astronomy, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Microorganisms. 2022 Apr 8;10(4):789. doi: 10.3390/microorganisms10040789.
The current work reports the biosynthesis of silver nanoparticles (AgNPs) using the antimicrobial activities of ethyl acetate extract of Urtica diocia (UD) leaves as a reducing and capping agent. The synthesized UD-AgNPs were characterized using UV−visible spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive X-ray analysis (EDAX), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), and dynamic light scattering (DLS). The UD-AgNPs were evaluated against Gram-positive and Gram-negative bacteria, and their size, shape, and distribution were recorded. The average size of an NP was 19.401 nm. The zone of inhibition (ZOI) for 75 µL of UD-AgNPs against Pseudomonas aeruginosa (P. aeruginosa) was 21 ± 0.4 mm more than that of the control drug Ciprofloxacin (16 ± 10 mm). The minimum inhibitory concentration (MIC) was the lowest against Escherichia coli (E. coli) (36 ± 3 µg/mL) and Staphylococcusepidermidis (S. epidermidis) (38 ± 3 µg/mL). Moreover, the minimum bactericidal concentration (MBC) was the lowest against E.coli (75 ± 00 µg/mL) and Enterococcus faecalis (E. faecalis (83 ± 16 µg/mL). Thus, the UD-AgNPs synthesized using the ethyl acetate extract of UD can be used as a new antimicrobial drug.
当前的研究报告了利用荨麻叶乙酸乙酯提取物的抗菌活性作为还原剂和封端剂来生物合成银纳米颗粒（AgNPs）。使用紫外可见光谱、扫描电子显微镜（SEM）、透射电子显微镜（TEM）、能量色散X射线分析（EDAX）、傅里叶变换红外（FTIR）光谱、X射线衍射（XRD）和动态光散射（DLS）对合成的UD-AgNPs进行了表征。对UD-AgNPs针对革兰氏阳性菌和革兰氏阴性菌进行了评估，并记录了它们的大小、形状和分布。纳米颗粒的平均大小为19.401纳米。75微升UD-AgNPs对铜绿假单胞菌（P. aeruginosa）的抑菌圈（ZOI）比对照药物环丙沙星（16±1.0毫米）大21±0.4毫米。最低抑菌浓度（MIC）对大肠杆菌（E. coli）（36±3微克/毫升）和表皮葡萄球菌（S. epidermidis）（38±3微克/毫升）最低。此外，最低杀菌浓度（MBC）对大肠杆菌（75±0.0微克/毫升）和粪肠球菌（E. faecalis）（83±1.6微克/毫升）最低。因此，使用UD乙酸乙酯提取物合成的UD-AgNPs可作为一种新型抗菌药物。
