College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China.
Department of Agronomy, Purdue University, West Lafayette, IN 47907, USA.
Int J Mol Sci. 2022 Apr 10;23(8):4185. doi: 10.3390/ijms23084185.
Zoysiagrass () is a popular turfgrass species and is widely used for sport turf and urban landscape. Zoysiagrass is often infected by , which causes a loss in turf quality. The physiological and molecular mechanisms of rust resistance are poorly understood in this species. In this study, the rust-resistant and susceptible lines of zoysiagrass were inoculated with , and alterations of leaf cell structure, physiological indicators and transcriptomic response were investigated at the various stages of inoculation. After inoculation, the cell membranes, nucleus, mitochondria, and chloroplast were all impaired, followed by abnormal physiological metabolism. The damage occurred earlier and more severely in the susceptible line. Changes in electrolyte leakage and chlorophyll content varied with the genotype and the inoculation stages. The transcriptome analysis showed that plant hormones, MAPK signal transduction pathway, photosynthesis and energy generation pathways were significantly enriched in the early response, in both the resistant and susceptible lines. The results provided insights into the physiological and molecular mechanisms of rust disease resistance and would benefit the breeding of rust-resistant varieties in zoysiagrass and related turfgrass species.
结缕草 () 是一种受欢迎的草坪草种，广泛用于运动草坪和城市景观。结缕草常受到锈病的感染，导致草坪质量下降。该物种对锈病抗性的生理和分子机制了解甚少。在这项研究中，接种了抗锈病和感病的结缕草品系，在接种的不同阶段研究了叶片细胞结构、生理指标和转录组应答的变化。接种后，细胞膜、细胞核、线粒体和叶绿体均受到损伤，随后出现异常的生理代谢。感病系的损伤发生得更早且更严重。电解质渗漏和叶绿素含量的变化随基因型和接种阶段而变化。转录组分析表明，在抗性和感病系中，植物激素、MAPK 信号转导途径、光合作用和能量产生途径在早期反应中显著富集。这些结果为锈病抗性的生理和分子机制提供了深入了解，将有助于结缕草和相关草坪草种锈病抗性品种的培育。
