College of Life Sciences, Northwest A&F University, Yangling 712100, China.
College of Landscape Architecture and Art, Northwest A&F University, Yangling 712100, China.
Int J Mol Sci. 2022 Apr 11;23(8):4219. doi: 10.3390/ijms23084219.
The growth of leaves is subject to strict time regulation. Several genes influencing leaf growth have been identified, but little is known about how genes regulate the orderly initiation and growth of leaves. Here, we demonstrate that contributes to a time regulation mechanism in leaves from initiation to expansion. encodes the cytochrome P450 CYP78A5, and its homolog has been described whose deletion is detrimental to organ growth. Our results show that overexpression increases leaf size and biomass by altering the time of leaf initiation and expansion. -overexpressing plants have larger leaves with more cells. Further dynamic observations indicate that enlarged wheat leaves have experienced a longer expansion time. Different from inactivation increases leaf number and initiation rates, overexpression only smooths the fluctuations of leaf initiation rates by adjusting the initiation time of local leaves, without affecting the overall leaf number and initiation rates. In addition, complementary analyses suggest is functionally conserved with in controlling the leaf initiation and size and may involve auxin accumulation. Our results provide a new insight into the time regulation mechanisms of leaf growth in wheat.
叶片的生长受到严格的时间调控。已经鉴定出了一些影响叶片生长的基因，但对于基因如何调控叶片有序的起始和生长还知之甚少。在这里，我们证明了 有助于从起始到扩展的叶片时间调控机制。 编码细胞色素 P450 CYP78A5，其同源物 已被描述，其缺失对器官生长有害。我们的结果表明，通过改变叶片起始和扩展的时间， 过表达增加了叶片的大小和生物量。 -过表达植株的叶片更大，细胞数量更多。进一步的动态观察表明，增大的小麦叶片经历了更长的扩展时间。与 失活增加叶片数量和起始率不同， 过表达仅通过调整局部叶片的起始时间来平滑叶片起始率的波动，而不影响总叶片数量和起始率。此外，互补分析表明 与 一样，在控制叶片起始和大小方面具有功能保守性，可能涉及生长素积累。我们的研究结果为小麦叶片生长的时间调控机制提供了新的见解。
