State Key Laboratory of Tree Genetics and Breeding, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100107, China.
Int J Mol Sci. 2023 Aug 30;24(17):13445. doi: 10.3390/ijms241713445.
Cell division plays an indispensable role in leaf morphogenesis, which is regulated via the complexes formed by cyclin and cyclin-dependent kinase (CDK). In this study, gene family analysis, exogenous auxin stimulation, RNA-seq and WGCNA analysis were all used to investigate the molecular mechanisms by which cell-cycle-related factors participated in the auxin signaling pathway on leaf morphogenesis. Sixty-three cyclin members and seventeen CDK members in were identified and systematically analyzed. During the evolution, WGD was the main reason that resulted in the expansion of and genes. Firstly, after a short time treating with auxin to matured leaves of seedlings, genes related to cell division including and were both upregulated to restart the transition of cells from G1-to-S phase. Secondly, with three days of continuous auxin stimulation to leaves at different developmental stages, leaves area variation, transcriptomes and hormones were analyzed. By PCA, PCoA and WGCNA analyses, the turquoise module was both positively related to leaf development and auxin. Based on the co-expression analysis and Y2H experiment, PoalbCYCD1;4, PoalbCYCD3;3 and PoalbCYCD3;5 were supposed to interact with PoalbCDKA;1, which could be the trigger to promote the G1-to-S phase transition. The ARF transcription factor might play the key role of connecting the auxin signaling pathway and cell division in leaf morphogenesis by affecting CYC-CDK complexes.
细胞分裂在叶片形态发生中起着不可或缺的作用，它通过细胞周期蛋白和细胞周期蛋白依赖性激酶（CDK）形成的复合物来调控。本研究采用基因家族分析、外源生长素刺激、RNA-seq 和 WGCNA 分析等方法，研究了细胞周期相关因子参与生长素信号通路对叶片形态发生的分子机制。鉴定并系统分析了拟南芥中的 63 个细胞周期蛋白成员和 17 个 CDK 成员。在进化过程中，WGD 是导致 和 基因扩张的主要原因。首先，在短时间用生长素处理幼苗的成熟叶片后，与细胞分裂相关的基因，包括 和 ，均上调以重新启动细胞从 G1 期到 S 期的转变。其次，对不同发育阶段叶片进行三天的连续生长素刺激，分析叶片面积变化、转录组和激素。通过 PCA、PCoA 和 WGCNA 分析，绿松石模块与叶片发育和生长素均呈正相关。基于共表达分析和 Y2H 实验，推测 PoalbCYCD1;4、PoalbCYCD3;3 和 PoalbCYCD3;5 与 PoalbCDKA;1 相互作用，可能是促进 G1 到 S 期转变的触发因素。ARF 转录因子可能通过影响 CYC-CDK 复合物，在生长素信号通路和叶片形态发生的细胞分裂中发挥关键作用。
