Department of Analytical Chemistry, Medical University of Bialystok, Mickiewicza 2D, 15-222 Bialystok, Poland.
Department of Infectious Diseases and Neuroinfections, Medical University of Bialystok, Zurawia 14, 15-540 Bialystok, Poland.
Int J Mol Sci. 2022 Apr 15;23(8):4374. doi: 10.3390/ijms23084374.
Despite the increasing number of patients suffering from tick-borne encephalitis (TBE), Lyme disease, and their co-infection, the mechanisms of the development of these diseases and their effects on the human body are still unknown. Therefore, the aim of this study was to evaluate the changes in the proteomic profile of human plasma induced by the development of TBE and to compare it with changes in TBE patients co-infected with other tick-borne pathogens. The results obtained by proteomic analysis using a nanoLC-Q Exactive HF mass spectrometer showed that the most highly elevated groups of proteins in the plasma of TBE patients with co-infection were involved in the pro-inflammatory response and protein degradation, while the antioxidant proteins and factors responsible for protein biosynthesis were mainly downregulated. These results were accompanied by enhanced GSH- and 4-HNE-protein adducts formation, observed in TBE and co-infected patients at a higher level than in the case of patients with only TBE. In conclusion, the differences in the proteomic profiles between patients with TBE and co-infected patients indicate that these diseases are significantly diverse and, consequently, require different treatment, which is particularly important for further research, including the development of novel diagnostics tools.
尽管患有蜱传脑炎（TBE）、莱姆病及其合并感染的患者数量不断增加，但这些疾病的发展机制及其对人体的影响仍不清楚。因此，本研究旨在评估 TBE 患者发展过程中诱导的人血浆蛋白质组图谱的变化，并将其与同时感染其他蜱传病原体的 TBE 患者的变化进行比较。使用 nanoLC-Q Exactive HF 质谱仪进行蛋白质组分析得到的结果表明，合并感染 TBE 患者血浆中高度升高的蛋白质组主要涉及炎症反应和蛋白质降解，而抗氧化蛋白和负责蛋白质生物合成的因子主要下调。这些结果伴随着增强的 GSH-和 4-HNE-蛋白质加合物形成，在 TBE 和合并感染患者中观察到的水平高于仅患有 TBE 的患者。总之，TBE 患者和合并感染患者之间蛋白质组图谱的差异表明这些疾病存在显著差异，因此需要不同的治疗方法，这对于进一步研究，包括开发新的诊断工具尤为重要。
