Department of Food Science and Human Nutrition, University of Veterinary & Animal Sciences, Lahore, Pakistan.
Department of Microbiology, University of Veterinary & Animal Sciences, Lahore, Pakistan.
Biomed Res Int. 2017;2017:8972678. doi: 10.1155/2017/8972678. Epub 2017 Aug 23.
-Glucan is a dietary fibre, found in many natural sources, and controls chronic metabolic diseases effectively. However, -glucan from the has rarely been investigated. Objectively, conditions were optimized to isolate -glucan from the yeast (max. 66% yield); those optimized conditions included 1.0 M NaOH, pH 7.0, and 90°C. The purity and identity of the isolated -glucan were characterized through FT-IR, SEM, DSC, and physicofunctional properties. The obtained results from DSC revealed highly stable -glucan (m.p., 125°C) with antioxidant activity (TAC value 0.240 ± 0.0021 g/mg, HO scavenging 38%), which has promising bile acid binding 40.463% and glucose control (in vitro). In line with these results, we evaluated the in vivo anti-inflammatory potential, that is, myeloperoxidase activity and reduction in MDA and NO; protective effect on proteins and keeping viscosity within normal range exhibited improvement. Also, the in vivo cholesterol binding and reduction in the skin thickness by -glucan were highly encouraging. Finally, our results confirmed that yeast -glucan is effective against some of the inflammatory and oxidative stress markers studied in this investigation. In general, the effect of 4% -glucan was more noticeable versus 2% -glucan. Therefore, our results support the utilization of -glucan as a novel, economically cheap, and functional food ingredient.
葡聚糖是一种膳食纤维，存在于许多天然来源中，可有效控制慢性代谢疾病。然而，酵母来源的 β-葡聚糖很少被研究过。本研究旨在客观地优化从酵母中分离β-葡聚糖的条件（最高产率 66%）；优化条件包括 1.0 M NaOH、pH7.0 和 90°C。采用傅里叶变换红外光谱（FT-IR）、扫描电子显微镜（SEM）、差示扫描量热法（DSC）和物理功能特性对分离得到的β-葡聚糖的纯度和结构进行了表征。DSC 获得的结果表明，β-葡聚糖具有高度稳定的结构（熔点 125°C）和抗氧化活性（TAC 值为 0.240±0.0021 g/mg，HO 清除率为 38%），具有潜在的结合胆汁酸 40.463%和控制葡萄糖的能力（体外）。根据这些结果，我们评估了其体内抗炎潜力，即髓过氧化物酶活性和 MDA 和 NO 的降低；对蛋白质的保护作用和维持正常范围内的粘度均有改善。此外，β-葡聚糖对体内胆固醇的结合作用以及对皮肤厚度的降低作用也非常令人鼓舞。最后，我们的结果证实，酵母β-葡聚糖对本研究中研究的一些炎症和氧化应激标志物具有治疗作用。一般来说，4%β-葡聚糖的效果比 2%β-葡聚糖更为显著。因此，我们的研究结果支持将β-葡聚糖作为一种新型、经济实惠且具有功能性的食品成分加以利用。
