Jia Jianjun, Zhu Wenjun, Liu Guodong, Diederich Sandra, Pickering Bradley, Banadyga Logan, Yang Ming
National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB R3E 3R2, Canada.
National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada.
Viruses. 2025 Jul 21;17(7):1021. doi: 10.3390/v17071021.
Nipah virus (NiV) and Hendra virus (HeV), which both belong to the genus henipavirus, are zoonotic pathogens that cause severe systemic, neurological, and/or respiratory disease in humans and a variety of mammals. Therefore, monitoring viral prevalence in natural reservoirs and rapidly diagnosing cases of henipavirus infection are critical to limiting the spread of these viruses. Current laboratory methods for detecting NiV and HeV include virus isolation, reverse transcription quantitative real-time PCR (RT-qPCR), and antigen detection via an enzyme-linked immunosorbent assay (ELISA), all of which require highly trained personnel and specialized equipment. Here, we describe the development of a point-of-care customized immunochromatographic lateral flow (ILF) assay that uses recombinant human ephrin B2 as a capture ligand on the test line and a NiV-specific monoclonal antibody (mAb) on the conjugate pad to detect NiV and HeV. The ILF assay detects NiV and HeV with a diagnostic specificity of 94.4% and has no cross-reactivity with other viruses. This rapid test may be suitable for field testing and in countries with limited laboratory resources.
尼帕病毒（NiV）和亨德拉病毒（HeV）均属于亨尼帕病毒属，是人畜共患病原体，可在人类和多种哺乳动物中引发严重的全身性、神经性和/或呼吸道疾病。因此，监测自然宿主中的病毒流行情况并快速诊断亨尼帕病毒感染病例对于限制这些病毒的传播至关重要。目前检测NiV和HeV的实验室方法包括病毒分离、逆转录定量实时PCR（RT-qPCR）以及通过酶联免疫吸附测定（ELISA）进行抗原检测，所有这些方法都需要训练有素的人员和专门的设备。在此，我们描述了一种即时检测定制免疫层析侧向流动（ILF）检测方法的开发，该方法在检测线上使用重组人ephrin B2作为捕获配体，在结合垫上使用NiV特异性单克隆抗体（mAb）来检测NiV和HeV。该ILF检测方法检测NiV和HeV的诊断特异性为94.4%，与其他病毒无交叉反应。这种快速检测方法可能适用于现场检测以及实验室资源有限的国家。
