• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

微小RNA-23b通过靶向干扰素调节因子1促进禽白血病病毒J亚群（ALV-J）复制。

MicroRNA-23b Promotes Avian Leukosis Virus Subgroup J (ALV-J) Replication by Targeting IRF1.

作者信息

Li Zhenhui, Chen Biao, Feng Min, Ouyang Hongjia, Zheng Ming, Ye Qiao, Nie Qinghua, Zhang Xiquan

机构信息

1] Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, China [2] Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, Guangdong, China.

出版信息

Sci Rep. 2015 May 18;5:10294. doi: 10.1038/srep10294.

DOI:10.1038/srep10294
PMID:25980475
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4434839/
Abstract

Avian leukosis virus subgroup J (ALV-J) can cause several different leukemia-like proliferative diseases in the hemopoietic system of chickens. Here, we investigated the transcriptome profiles and miRNA expression profiles of ALV-J-infected and uninfected chicken spleens to identify the genes and miRNAs related to ALV-J invasion. In total, 252 genes and 167 miRNAs were differentially expressed in ALV-J-infected spleens compared to control uninfected spleens. miR-23b expression was up-regulated in ALV-J-infected spleens compared with the control spleens, and transcriptome analysis revealed that the expression of interferon regulatory factor 1 (IRF1) was down-regulated in ALV-J-infected spleens compared to uninfected spleens. A dual-luciferase reporter assay showed that IRF1 was a direct target of miR-23b. miR-23b overexpression significantly (P = 0.0022) decreased IRF1 mRNA levels and repressed IRF1-3'-UTR reporter activity. In vitro experiments revealed that miR-23b overexpression strengthened ALV-J replication, whereas miR-23b loss of function inhibited ALV-J replication. IRF1 overexpression inhibited ALV-J replication, and IRF1 knockdown enhanced ALV-J replication. Moreover, IRF1 overexpression significantly (P = 0.0014) increased IFN-β expression. In conclusion, these results suggested that miR-23b may play an important role in ALV-J replication by targeting IRF1.

摘要

禽白血病病毒J亚群（ALV-J）可在鸡的造血系统中引发几种不同的白血病样增殖性疾病。在此，我们研究了ALV-J感染和未感染鸡脾脏的转录组图谱和miRNA表达谱，以鉴定与ALV-J入侵相关的基因和miRNA。与未感染的对照脾脏相比，ALV-J感染的脾脏中共有252个基因和167个miRNA差异表达。与对照脾脏相比，miR-23b在ALV-J感染的脾脏中表达上调，转录组分析显示，与未感染的脾脏相比，干扰素调节因子1（IRF1）在ALV-J感染的脾脏中表达下调。双荧光素酶报告基因检测表明IRF1是miR-23b的直接靶标。miR-23b过表达显著（P = 0.0022）降低IRF1 mRNA水平并抑制IRF1-3'-UTR报告基因活性。体外实验表明，miR-23b过表达增强了ALV-J复制，而miR-23b功能缺失则抑制了ALV-J复制。IRF1过表达抑制ALV-J复制，而IRF1敲低增强ALV-J复制。此外，IRF1过表达显著（P = 0.0014）增加IFN-β表达。总之，这些结果表明miR-23b可能通过靶向IRF1在ALV-J复制中发挥重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f452/4434839/6fee2ae19d8f/srep10294-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f452/4434839/f573a7b1d2f0/srep10294-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f452/4434839/239f54150d74/srep10294-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f452/4434839/bd8be8e167a6/srep10294-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f452/4434839/d6649f9c7ec2/srep10294-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f452/4434839/35c101dc6dea/srep10294-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f452/4434839/0da5c5eaacb7/srep10294-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f452/4434839/6fee2ae19d8f/srep10294-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f452/4434839/f573a7b1d2f0/srep10294-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f452/4434839/239f54150d74/srep10294-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f452/4434839/bd8be8e167a6/srep10294-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f452/4434839/d6649f9c7ec2/srep10294-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f452/4434839/35c101dc6dea/srep10294-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f452/4434839/0da5c5eaacb7/srep10294-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f452/4434839/6fee2ae19d8f/srep10294-f7.jpg

相似文献

1
MicroRNA-23b Promotes Avian Leukosis Virus Subgroup J (ALV-J) Replication by Targeting IRF1.微小RNA-23b通过靶向干扰素调节因子1促进禽白血病病毒J亚群（ALV-J）复制。
Sci Rep. 2015 May 18;5:10294. doi: 10.1038/srep10294.
2
MiR-34b-5p Suppresses Melanoma Differentiation-Associated Gene 5 () Signaling Pathway to Promote Avian Leukosis Virus Subgroup J (ALV-J)-Infected Cells Proliferaction and ALV-J Replication.微小RNA-34b-5p抑制黑色素瘤分化相关基因5（MDA5）信号通路以促进禽白血病病毒J亚群（ALV-J）感染细胞的增殖及ALV-J复制。
Front Cell Infect Microbiol. 2017 Jan 30;7:17. doi: 10.3389/fcimb.2017.00017. eCollection 2017.
3
Growth retardation induced by avian leukosis virus subgroup J associated with down-regulated Wnt/β-catenin pathway.J亚群禽白血病病毒诱导的生长迟缓与Wnt/β-连环蛋白信号通路下调有关。
Microb Pathog. 2017 Mar;104:48-55. doi: 10.1016/j.micpath.2017.01.013. Epub 2017 Jan 5.
4
Subgroup J avian leukosis virus infection of chicken dendritic cells induces apoptosis via the aberrant expression of microRNAs.J亚群禽白血病病毒感染鸡树突状细胞通过微小RNA的异常表达诱导细胞凋亡。
Sci Rep. 2016 Feb 1;6:20188. doi: 10.1038/srep20188.
5
Integrated host and viral transcriptome analyses reveal pathology and inflammatory response mechanisms to ALV-J injection in SPF chickens.整合宿主和病毒转录组分析揭示了 SPF 鸡中 ALV-J 注射的病理学和炎症反应机制。
Sci Rep. 2017 Apr 12;7:46156. doi: 10.1038/srep46156.
6
Synergistic inhibition of avian leukosis virus subgroup J replication by miRNA-embedded siRNA interference of double-target.通过双靶点miRNA嵌入的siRNA干扰协同抑制禽白血病病毒J亚群复制
Virol J. 2015 Mar 21;12:45. doi: 10.1186/s12985-015-0277-5.
7
Aberrant expression of liver microRNA in chickens infected with subgroup J avian leukosis virus.亚群 J 禽白血病病毒感染鸡肝脏 microRNA 的异常表达。
Virus Res. 2012 Oct;169(1):268-71. doi: 10.1016/j.virusres.2012.07.003. Epub 2012 Jul 16.
8
Viral proliferation and expression of tumor-related gene in different chicken embryo fibroblasts infected with different tumorigenic phenotypes of avian leukosis virus subgroup J.不同致瘤表型的J亚群禽白血病病毒感染不同鸡胚成纤维细胞后病毒增殖及肿瘤相关基因的表达
Poult Sci. 2016 Oct 1;95(10):2383-90. doi: 10.3382/ps/pew180. Epub 2016 Jun 9.
9
Avian leukosis virus subgroup J promotes cell proliferation and cell cycle progression through miR-221 by targeting CDKN1B.禽白血病病毒 J 亚群通过靶向 CDKN1B 促进 miR-221 的表达从而促进细胞增殖和细胞周期进程。
Virology. 2018 Jun;519:121-130. doi: 10.1016/j.virol.2018.04.008. Epub 2018 May 11.
10
Differential expression of immune-related cytokine genes in response to J group avian leukosis virus infection in vivo.免疫相关细胞因子基因在体内对 J 群禽白血病病毒感染的差异表达。
Mol Immunol. 2015 Mar;64(1):106-11. doi: 10.1016/j.molimm.2014.11.004. Epub 2014 Nov 29.

引用本文的文献

1
Autophagy-mediated TET2 degradation by ALV-J Env protein suppresses innate immune activation to promote viral replication.禽白血病病毒J亚群（ALV-J）Env蛋白通过自噬介导的TET2降解抑制先天性免疫激活以促进病毒复制。
J Virol. 2025 Feb 25;99(2):e0200324. doi: 10.1128/jvi.02003-24. Epub 2025 Jan 22.
2
MiRNAs and lncRNAs in the regulation of innate immune signaling.微小RNA和长链非编码RNA在先天免疫信号调节中的作用
Noncoding RNA Res. 2023 Aug 1;8(4):534-541. doi: 10.1016/j.ncrna.2023.07.002. eCollection 2023 Dec.
3
Avian Leukosis: Will We Be Able to Get Rid of It?

本文引用的文献

1
MicroRNA-296-5p (miR-296-5p) functions as a tumor suppressor in prostate cancer by directly targeting Pin1.微小RNA-296-5p（miR-296-5p）通过直接靶向肽基脯氨酰顺反异构酶NIMA相互作用蛋白1（Pin1）在前列腺癌中发挥肿瘤抑制作用。
Biochim Biophys Acta. 2014 Sep;1843(9):2055-66. doi: 10.1016/j.bbamcr.2014.06.001. Epub 2014 Jun 8.
2
MicroRNA-135b promotes cancer progression by acting as a downstream effector of oncogenic pathways in colon cancer.微小 RNA-135b 通过作为结肠癌致癌途径的下游效应物促进癌症进展。
Cancer Cell. 2014 Apr 14;25(4):469-83. doi: 10.1016/j.ccr.2014.03.006.
3
gga-miR-375 plays a key role in tumorigenesis post subgroup J avian leukosis virus infection.
禽白血病：我们能根除它吗？
Animals (Basel). 2023 Jul 19;13(14):2358. doi: 10.3390/ani13142358.
4
Optimization of cancer immunotherapy on the basis of programmed death ligand-1 distribution and function.基于程序性死亡配体-1 分布和功能的癌症免疫治疗优化。
Br J Pharmacol. 2024 Jan;181(2):257-272. doi: 10.1111/bph.16054. Epub 2023 Mar 1.
5
A comprehensive analysis of avian lymphoid leukosis-like lymphoma transcriptomes including identification of LncRNAs and the expression profiles.对禽类淋巴白血病样淋巴瘤转录组进行全面分析，包括鉴定 LncRNAs 和表达谱。
PLoS One. 2022 Aug 8;17(8):e0272557. doi: 10.1371/journal.pone.0272557. eCollection 2022.
6
Avian Leucosis Virus-Host Interaction: The Involvement of Host Factors in Viral Replication.禽类白细胞增生症病毒-宿主相互作用：宿主因子在病毒复制中的参与。
Front Immunol. 2022 May 26;13:907287. doi: 10.3389/fimmu.2022.907287. eCollection 2022.
7
Advances on Innate Immune Evasion by Avian Immunosuppressive Viruses.禽类免疫抑制病毒的先天免疫逃避进展。
Front Immunol. 2022 May 12;13:901913. doi: 10.3389/fimmu.2022.901913. eCollection 2022.
8
MicroRNA-26a-3p rescues depression-like behaviors in male rats via preventing hippocampal neuronal anomalies.miRNA-26a-3p 通过防止海马神经元异常来挽救雄性大鼠的抑郁样行为。
J Clin Invest. 2021 Aug 16;131(16). doi: 10.1172/JCI148853.
9
miRNA Regulatory Functions in Farm Animal Diseases, and Biomarker Potentials for Effective Therapies.miRNA 在农场动物疾病中的调控功能，以及有效治疗的生物标志物潜力。
Int J Mol Sci. 2021 Mar 17;22(6):3080. doi: 10.3390/ijms22063080.
10
MicroRNAs as Biomarkers for Animal Health and Welfare in Livestock.微小RNA作为家畜动物健康和福利的生物标志物
Front Vet Sci. 2020 Dec 18;7:578193. doi: 10.3389/fvets.2020.578193. eCollection 2020.
gga-miR-375 在 J 亚群禽白血病病毒感染后的肿瘤发生中发挥关键作用。
PLoS One. 2014 Apr 2;9(4):e90878. doi: 10.1371/journal.pone.0090878. eCollection 2014.
4
IFNβ-dependent increases in STAT1, STAT2, and IRF9 mediate resistance to viruses and DNA damage.IFNβ依赖性增加的 STAT1、STAT2 和 IRF9 介导对病毒和 DNA 损伤的抗性。
EMBO J. 2013 Oct 16;32(20):2751-63. doi: 10.1038/emboj.2013.203. Epub 2013 Sep 24.
5
The imprinted H19 lncRNA antagonizes let-7 microRNAs.印记的 H19 lncRNA 拮抗 let-7 微 RNA。
Mol Cell. 2013 Oct 10;52(1):101-12. doi: 10.1016/j.molcel.2013.08.027. Epub 2013 Sep 19.
6
Overexpression of microRNA gga-miR-1650 decreases the replication of avian leukosis virus subgroup J in infected cells.gga-miR-1650 的过表达降低了感染细胞中 J 亚群禽白血病病毒的复制。
J Gen Virol. 2013 Oct;94(Pt 10):2287-2296. doi: 10.1099/vir.0.054007-0. Epub 2013 Aug 1.
7
miR-23a targets interferon regulatory factor 1 and modulates cellular proliferation and paclitaxel-induced apoptosis in gastric adenocarcinoma cells.miR-23a 靶向干扰素调节因子 1 并调节胃腺癌细胞的增殖和紫杉醇诱导的细胞凋亡。
PLoS One. 2013 Jun 10;8(6):e64707. doi: 10.1371/journal.pone.0064707. Print 2013.
8
MicroRNA-29c functions as a tumor suppressor by direct targeting oncogenic SIRT1 in hepatocellular carcinoma.MicroRNA-29c 通过直接靶向肝癌中的致癌 SIRT1 发挥肿瘤抑制作用。
Oncogene. 2014 May 15;33(20):2557-67. doi: 10.1038/onc.2013.216. Epub 2013 Jun 3.
9
Smad7 protein induces interferon regulatory factor 1-dependent transcriptional activation of caspase 8 to restore tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis.Smad7 蛋白诱导干扰素调节因子 1 依赖性 caspase 8 的转录激活，从而恢复肿瘤坏死因子相关凋亡诱导配体（TRAIL）介导的细胞凋亡。
J Biol Chem. 2013 Feb 1;288(5):3560-70. doi: 10.1074/jbc.M112.400408. Epub 2012 Dec 19.
10
Aberrant expression of liver microRNA in chickens infected with subgroup J avian leukosis virus.亚群 J 禽白血病病毒感染鸡肝脏 microRNA 的异常表达。
Virus Res. 2012 Oct;169(1):268-71. doi: 10.1016/j.virusres.2012.07.003. Epub 2012 Jul 16.