Faculty of Food Science and Nutrition, Universiti Malaysia Sabah, Kota Kinabalu 88400, Malaysia.
Faculty of Fisheries and Marine Science, Universitas Brawijaya, Malang 65145, Indonesia.
Molecules. 2022 Apr 11;27(8):2452. doi: 10.3390/molecules27082452.
Marine fish collagen has attracted considerable attention due to its characteristics, including its biodegradability, biocompatibility, and weak antigenicity, and is considered a safer material compared to collagen from terrestrial animals. The aim of this study was to extract and characterize collagen from the skin of lizardfish ( Bloch, 1795) with three different acids. The yields of acetic acid-extracted collagen (AESkC), lactic acid-extracted collagen (LESkC), and citric acid-extracted collagen (CESkC) were 11.73 ± 1.14%, 11.63 ± 1.10%, and 11.39 ± 1.05% (based on wet weight), respectively. All extracted collagens were categorized as type I collagen with mainly alpha chains (α and α) detected and γ and β chains to some extent. Fourier transform infrared (FTIR) spectra showed an intact triple-helical structure in the AESkC, LESkC, and CESkC. UV-vis spectra and X-ray diffraction further demonstrated the similarity of the extracted collagens to previously reported fish skin collagens. AESkC ( = 40.24 °C) had higher thermostability compared to LESkC ( = 38.72 °C) and CESkC ( = 36.74 °C). All samples were highly soluble in acidic pH and low concentrations of NaCl (0-20 g/L). Under field emission scanning electron microscopy (FESEM) observation, we noted the loose, fibrous, and porous structures of the collagens. The results suggest that the lizardfish skin collagens could be a potential alternative source of collagen, especially the AESkC due to its greater thermostability characteristic.
鱼皮胶原蛋白因其可生物降解性、生物相容性和低抗原性等特点而备受关注，被认为是比陆生动物胶原蛋白更安全的材料。本研究旨在采用三种不同酸从圆斑星鲽（Bloch，1795）皮中提取和表征胶原蛋白。乙酸提取的胶原蛋白（AESkC）、乳酸提取的胶原蛋白（LESkC）和柠檬酸提取的胶原蛋白（CESkC）的得率分别为 11.73±1.14%、11.63±1.10%和 11.39±1.05%（基于湿重）。所有提取的胶原蛋白均归类为 I 型胶原蛋白，主要检测到α链（α和α），并在一定程度上检测到γ和β链。傅里叶变换红外（FTIR）光谱显示 AESkC、LESkC 和 CESkC 中均存在完整的三螺旋结构。紫外-可见光谱和 X 射线衍射进一步表明，提取的胶原蛋白与先前报道的鱼类皮胶原蛋白具有相似性。AESkC（ = 40.24°C）的热稳定性高于 LESkC（ = 38.72°C）和 CESkC（ = 36.74°C）。所有样品在酸性 pH 值和低浓度 NaCl（0-20 g/L）中均具有高溶解性。场发射扫描电子显微镜（FESEM）观察表明，胶原蛋白具有疏松、纤维状和多孔的结构。结果表明，圆斑星鲽皮胶原蛋白可能是胶原蛋白的潜在替代来源，特别是 AESkC，因为其具有更高的热稳定性特性。
