基于紫外分光光度法的便携式设备对尿液进行无标记尿酸估计。

Taiwan RedEye Biomedical Inc., Hsinchu City 300, Taiwan.

Department of Electrophysics, National Yang Ming Chiao Tung University, Hsinchu City 300093, Taiwan.

Sensors (Basel). 2022 Apr 14;22(8):3009. doi: 10.3390/s22083009.

The maintenance of uric acid levels is crucial for the human body. In this study, the feasibility of using portable ultraviolet (UV) spectrophotometry to measure the uric acid of spot urine without the need to add reagents has been demonstrated for the first time. UV spectral analysis has been used to inspect the uric acid concentration in urine. It is found that the absorption spectrum of urine has a high correlation with the concentration of uric acid at a wavelength of around 290-300 nm. Uric acid levels measured with a spectral analyzer compared to uric acid concentrations measured with a traditional biochemical analysis showed good agreement. The portable prototype is label-free and capable of displaying the inspection result of each measurement within 10 s. In the long run, this device can assist people in checking uric acid levels of spot urine with higher frequency and can adjust diet or medication in real time for more efficient health management.

尿酸水平的维持对人体至关重要。本研究首次证明，无需添加试剂即可使用便携式紫外（UV）分光光度法测量尿样中的尿酸是可行的。利用 UV 光谱分析来检测尿液中的尿酸浓度。结果发现，在波长约 290-300nm 处，尿液的吸收光谱与尿酸浓度具有高度相关性。与传统生化分析测量的尿酸浓度相比，光谱分析仪测量的尿酸水平具有良好的一致性。该便携式原型设备无标记，可在 10s 内显示每次测量的检查结果。从长远来看，该设备可以帮助人们更频繁地检查尿样中的尿酸水平，并实时调整饮食或药物，以实现更高效的健康管理。