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5种正品药材多指标的熵值分析与灰色聚类分析

Entropy analysis and grey cluster analysis of multiple indexes of 5 kinds of genuine medicinal materials.

作者信息

Zhou Libing, Jiang Caiyun, Lin Qingxia

机构信息

Guangxi Science & Technology Normal University, Laibin, 546199, Guangxi, China.

出版信息

Sci Rep. 2022 Apr 22;12(1):6618. doi: 10.1038/s41598-022-10509-0.

Abstract

5 kinds of genuine medicinal materials, including Diding (Latin name: Corydalis bungeana Turcz), Purslane (Latin name: Portulaca oleracea L.), straw sandal board (Latin name: Hoya carnosa (L.f.) R. Br), June snow (Latin name: Serissa japonica (Thunb.) Thunb.), pine vine rattan (Latin name: Lycopodiastrum casuarinoides (Spring) Holub. [Lycopodium casuarinoides Spring]), were selected as the research objects. The combustion heat, thermo gravimetric parameters, and fat content, calcium content, trace element content, ash content of 5 kinds of genuine medicinal materials were measured. The combustion heat, differential thermal gravimetric analysis, fat content, calcium content, trace elements content, and ash content of 5 kinds of genuine medicinal materials were used to build a systematic multi-index evaluation system by gray pattern recognition and grey correlation coefficient cluster analysis, which can make up for the gaps in this area and provide scientific basis and research significance for the study of genuine medicinal materials quality. The results showed that the order of combustion heat of 5 kinds of genuine medicinal materials, including Diding, Purslane, straw sandal board, June snow, pine vine rattan, was Diding > June snow > straw sandal board > Purslane > pine vine rattan, the order of fat content (%) of 5 kinds of genuine medicinal materials was straw sandal board > Diding > pine vine rattan > June snow > Purslane, the order of calcium content (%) was pine vine rattan > June snow > Purslane > straw sandal board > Diding, the order of ash content was June snow > Purslane > straw sandal board > pine vine rattan > Diding. From the analysis of thermogravimetric analysis results and thermogravimetric combustion stability, the order of combustion stability of 5 kinds of genuine medicinal materials was June snow > pine Vine rattan > straw sandal board > Diding > Portulaca oleracea. The order of the content of 12 trace elements in 5 kinds of genuine medicinal materials, in terms of trace element content, June snow contains the highest trace elements in all samples. According to combustion heat, combustibility (combustion stability of genuine medicinal materials), fat, calcium, ash, trace element content, the comprehensive evaluation results of multi-index analysis constructed by gray correlation degree, gray correlation coefficient factor analysis, and gray hierarchical cluster analysis showed that the comprehensive evaluation multi-index order of 5 genuine medicinal materials, including Diding, Purslane, straw sandal board, June snow and pine vine rattan, was June snow > straw sandal board > Diding > Purslane > pine vine rattan. Therefore, the comprehensive evaluation results of the quality of genuine medicinal materials selected in this study were June snow the best, followed by straw sandal board. This research has important theoretical and practical significance for the multi-index measurement and comprehensive evaluation of genuine medicinal materials, and can provide scientific basis and research significance for the research of multi-index quality control of genuine medicinal material.

摘要

选取了5种道地药材,包括地丁(拉丁名:Corydalis bungeana Turcz)、马齿苋(拉丁名:Portulaca oleracea L.)、络石藤(拉丁名:Hoya carnosa (L.f.) R. Br)、六月雪(拉丁名:Serissa japonica (Thunb.) Thunb.)、垂穗石松(拉丁名:Lycopodiastrum casuarinoides (Spring) Holub. [Lycopodium casuarinoides Spring])作为研究对象。测定了5种道地药材的燃烧热、热重参数以及脂肪含量、钙含量、微量元素含量、灰分含量。利用5种道地药材的燃烧热、差示热重分析、脂肪含量、钙含量、微量元素含量和灰分含量,通过灰色模式识别和灰色关联度聚类分析构建了系统的多指标评价体系,弥补了该领域的空白,为道地药材质量研究提供了科学依据和研究意义。结果表明,地丁、马齿苋、络石藤、六月雪、垂穗石松这5种道地药材的燃烧热顺序为地丁>六月雪>络石藤>马齿苋>垂穗石松;5种道地药材的脂肪含量(%)顺序为络石藤>地丁>垂穗石松>六月雪>马齿苋;钙含量(%)顺序为垂穗石松>六月雪>马齿苋>络石藤>地丁;灰分含量顺序为六月雪>马齿苋>络石藤>垂穗石松>地丁。从热重分析结果和热重燃烧稳定性分析来看,5种道地药材的燃烧稳定性顺序为六月雪>垂穗石松>络石藤>地丁>马齿苋。5种道地药材中12种微量元素的含量,就微量元素含量而言,六月雪在所有样品中所含微量元素最高。根据燃烧热、燃烧性(道地药材的燃烧稳定性)、脂肪、钙、灰分、微量元素含量,通过灰色关联度、灰色关联系数因子分析和灰色层次聚类分析构建的多指标分析综合评价结果表明,地丁、马齿苋、络石藤、六月雪、垂穗石松这5种道地药材的综合评价多指标顺序为六月雪>络石藤>地丁>马齿苋>垂穗石松。因此,本研究选取的道地药材质量综合评价结果为六月雪最佳,其次是络石藤。本研究对于道地药材的多指标测定和综合评价具有重要的理论和实践意义,可为道地药材多指标质量控制研究提供科学依据和研究意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de7e/9033816/4a24fc2a0fbf/41598_2022_10509_Fig1_HTML.jpg

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