洋葱多糖的提取、衍生化及抗氧化活性
Extraction, derivatization and antioxidant activities of onion polysaccharide.
作者信息
Zhou Shiyang, Huang Gangliang, Huang Hualiang
机构信息
Chongqing Chemical Industry Vocational College, Chongqing 401220, China; Laboratory of Carbohydrate Science and Engineering, Chongqing Key Laboratory of Inorganic Functional Materials, Chongqing Normal University, Chongqing 401331, China.
Laboratory of Carbohydrate Science and Engineering, Chongqing Key Laboratory of Inorganic Functional Materials, Chongqing Normal University, Chongqing 401331, China.
出版信息
Food Chem. 2022 Sep 15;388:133000. doi: 10.1016/j.foodchem.2022.133000. Epub 2022 Apr 20.
The preparation of purified polysaccharides was described from fresh onions by hot water extraction, protein removal and dialysis treatment. The structure of onion polysaccharide was confirmed by C NMR, DEPT, COSY, HSQC and HMBC. In order to study the structure-activity relationship of onion polysaccharide, the derivatives of onion polysaccharide including acetylated onion polysaccharide and phosphoric onion polysaccharide were prepared. On this basis, the antioxidant activities of onion polysaccharide and its derivatives were tested in vitro. The results showed that onion polysaccharides and its derivatives had a good antioxidant activity, and the activity of phosphorylated polysaccharide was similar to that of V positive control.
本文描述了从新鲜洋葱中通过热水提取、去除蛋白质和透析处理制备纯化多糖的方法。通过碳核磁共振（C NMR）、无畸变极化转移增强法（DEPT）、同核化学位移相关谱（COSY）、异核单量子相干谱（HSQC）和异核多键相关谱（HMBC）确定了洋葱多糖的结构。为了研究洋葱多糖的构效关系，制备了洋葱多糖的衍生物，包括乙酰化洋葱多糖和磷酸化洋葱多糖。在此基础上，体外测试了洋葱多糖及其衍生物的抗氧化活性。结果表明，洋葱多糖及其衍生物具有良好的抗氧化活性，磷酸化多糖的活性与维生素C阳性对照相似。
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