通过酶解甘蔗渣和椰壳纤维生产低聚木糖的比较分析
A Comparative Analysis for the Production of Xylooligosaccharides via Enzymatic Hydrolysis from Sugarcane Bagasse and Coconut Coir.
作者信息
Khangwal Ishu, Shukla Pratyoosh
机构信息
Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak, Haryana 124001 India.
Present Address: School of Biotechnology, Institute of Science, Banaras Hindu University, Varanasi, 221005 India.
出版信息
Indian J Microbiol. 2022 Jun;62(2):317-321. doi: 10.1007/s12088-022-01010-3. Epub 2022 Mar 29.
UNLABELLED
Xylooligosaccharides are known prebiotics that various foods and feed industries can utilize. In the present study, the xylan was extracted from sugarcane bagasse and coconut coir by NaOH treatment and it was further structurally characterized by FTIR. Furthermore, VAPS-24 was used to produce endo-xylanase, which induced the production of XOS from both of these substrates. The maximum production of XOS in 8 h using 20U enzyme concentration and 2% substrate concentration was found as 10.10 mg/ml, 5.43 mg/ml for sugarcane bagasse and coconut coir, respectively. Further, the produced XOS was structurally characterized by the NMR, HPLC and FTIR analysis. This study gives a clue that the sugarcane bagasse can be used as the potent producer for the xylooligosaccharides as compared to the coconut coir and can be utilized at the industrial level.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s12088-022-01010-3.
未标注
低聚木糖是已知的益生元，各种食品和饲料行业都可加以利用。在本研究中，通过氢氧化钠处理从甘蔗渣和椰壳中提取木聚糖，并通过傅里叶变换红外光谱（FTIR）对其结构进行进一步表征。此外，使用VAPS - 24生产内切木聚糖酶，该酶可从这两种底物诱导产生低聚木糖。发现使用20U酶浓度和2%底物浓度在8小时内低聚木糖的最大产量分别为：甘蔗渣10.10毫克/毫升，椰壳5.43毫克/毫升。此外，通过核磁共振（NMR）、高效液相色谱（HPLC）和傅里叶变换红外光谱（FTIR）分析对所产生的低聚木糖进行结构表征。本研究表明，与椰壳相比，甘蔗渣可作为低聚木糖的有效生产原料，并可在工业层面加以利用。
补充信息
在线版本包含可在10.1007/s12088 - 022 - 01010 - 3获取的补充材料。
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