高氯酸血竭红素通过TLR4通路调节炎性细胞因子表达并改善糖尿病大鼠皮肤伤口愈合
Dracorhodin Perchlorate Regulates the Expression of Inflammatory Cytokines through the TLR4 Pathway and Improves Skin Wound Healing in Diabetic Rats.
作者信息
Xiong Zongliang, Huo Mohan, Jia Yongzhen, Zhou Chong, Ma Xianglin, Yin Hang, Jiang Xiaowen, Yu Wenhui
机构信息
Department of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, China.
Department of Life Sciences, Northeast Agricultural University, Harbin, Heilongjiang, China.
出版信息
Evid Based Complement Alternat Med. 2022 Apr 14;2022:9050686. doi: 10.1155/2022/9050686. eCollection 2022.
BACKGROUND
Dragon's blood is a natural medicine with hemostatic and blood-activating effects and is used to promote wound healing. Dracorhodin perchlorate (DP) is a stable form of dracarhod and is used as a substitute for cochinchinenin. DP promotes the proliferation of rat fibroblasts and promotes wound healing in rats.
METHODS
DP ointment (0.2 mg/mL) was applied to the skin wounds of nondiabetic and diabetic rats, and the skin of the wound was collected. Wound healing rate, H&E staining, Masson staining, TLR4 pathway, related inflammatory factors, nitric oxide synthase, and so forth were detected.
RESULTS
DP treatment alleviated the prolonged inflammatory cell infiltration time and the increase in the TLR4 pathway and inflammatory factors caused by diabetes. DP also promoted wound healing by increasing eNOS protein expression and NO content in the later stage of wound healing.
CONCLUSION
DP promotes wound healing in diabetic rats by regulating the TLR4 pathway and related inflammatory factors. Therefore, adjuvant treatment of DP can be developed for diabetic wound healing.
背景
血竭是一种具有止血和活血作用的天然药物，用于促进伤口愈合。高氯血竭素（DP）是血竭红素的一种稳定形式，用作儿茶素的替代品。DP可促进大鼠成纤维细胞增殖并促进大鼠伤口愈合。
方法
将DP软膏（0.2mg/mL）应用于非糖尿病和糖尿病大鼠的皮肤伤口，并收集伤口皮肤。检测伤口愈合率、苏木精-伊红染色、马松染色、TLR4通路、相关炎症因子、一氧化氮合酶等。
结果
DP治疗减轻了糖尿病引起的炎症细胞浸润时间延长以及TLR4通路和炎症因子的增加。DP还通过增加伤口愈合后期的内皮型一氧化氮合酶蛋白表达和一氧化氮含量来促进伤口愈合。
结论
DP通过调节TLR4通路和相关炎症因子促进糖尿病大鼠伤口愈合。因此，可开发DP辅助治疗糖尿病伤口愈合。
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