Yin Ge, Wang Jingyue, Wang Xiao, Zhan Yu, Tang Xuegui, Wu Qie, Wang Xian, Du Lijuan, Lu Xiong
Department of Anorectal, Affiliated Hospital of Southwest Jiaotong University, Chengdu, China.
Department of Anorectal, Chengdu Thrid People's Hospital, Chengdu, China.
Front Bioeng Biotechnol. 2022 Sep 30;10:989180. doi: 10.3389/fbioe.2022.989180. eCollection 2022.
Postoperative wound of perianal infectious disease represents a common but unique refractory wound in clinical practice. The reasons that hinder the wound healing process include not only the severe bacterial infection of the wound itself and the narrow and deep shape of the wound, but also its frequent bacterial contact. Therefore, the development of biofunctional dressings to aid in therapy is essential. In this study, we synthesized a new type of dressing comprising a hydrogel host based on the Schiff base principle and catechol groups between polydopamine, oxidized dextran, and quaternized chitosan, and then loaded it with traditional Chinese medicine molecules. These formed an integrated hydrogel for accelerated wound repair in a perianal infection model. The prepared hydrogels exhibited excellent wet tissue adhesion, antifouling, morphological variability, suitable swelling properties, and complete degradability, as well as remarkable contact antibacterial ability and the ability to rapidly scavenge free radicals. Hemostatic experiments showed excellent hemostatic properties, as the integrated hydrogel could instantly gel to seal the hemorrhage. Hemocompatibility and cell experiments showed that the integrated hydrogel had good biosafety and significantly promoted cell proliferation, which in turn accelerated the repair of infected whole cortexes in rats. A histomorphological evaluation showed that the integrated hydrogel promoted the recovery of normal anatomical tissue in rats by promoting the formation of collagen fibers and inhibiting inflammation. The results showed that this multifunctional integrated hydrogel has great potential for the treatment of continuously infected skin regeneration, providing a promising therapeutic strategy for postoperative wound healing in perianal infectious diseases.
肛周感染性疾病术后创面是临床常见但独特的难治性创面。阻碍创面愈合的原因不仅包括创面本身严重的细菌感染、创面狭窄且深的形状，还包括其频繁的细菌接触。因此，开发有助于治疗的生物功能敷料至关重要。在本研究中，我们基于席夫碱原理合成了一种新型敷料，其水凝胶主体包含聚多巴胺、氧化葡聚糖和季铵化壳聚糖之间的儿茶酚基团，然后负载中药分子。这些形成了一种集成水凝胶，用于在肛周感染模型中加速创面修复。制备的水凝胶表现出优异的湿组织粘附性、抗污性、形态可变性、合适的溶胀性能和完全可降解性，以及显著的接触抗菌能力和快速清除自由基的能力。止血实验显示出优异的止血性能，因为集成水凝胶能立即凝胶化以封闭出血。血液相容性和细胞实验表明，集成水凝胶具有良好的生物安全性，并显著促进细胞增殖，进而加速大鼠感染全皮层的修复。组织形态学评估表明，集成水凝胶通过促进胶原纤维形成和抑制炎症来促进大鼠正常解剖组织的恢复。结果表明，这种多功能集成水凝胶在治疗持续感染的皮肤再生方面具有巨大潜力，为肛周感染性疾病术后创面愈合提供了一种有前景的治疗策略。
