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用针对ras癌基因p21产物和碳水化合物决定簇抗原19-9的单克隆抗体对结肠直肠组织进行免疫组织化学染色。

Immunohistochemical staining of colorectal tissues with monoclonal antibodies to ras oncogene p21 product and carbohydrate determinant antigen 19-9.

作者信息

Allen D C, Foster H, Orchin J C, Biggart J D

出版信息

J Clin Pathol. 1987 Feb;40(2):157-62. doi: 10.1136/jcp.40.2.157.

DOI:10.1136/jcp.40.2.157
PMID:3546394
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1140859/
Abstract

Two monoclonal antibodies were applied to benign, dysplastic, and malignant human colorectal tissues using immunohistochemical techniques on formalin fixed paraffin embedded material. RAP-5 antibody is directed against a synthetic peptide, reflecting an amino acid sequence of the ras oncogene p21 protein product. Despite using several different techniques and antibody dilutions differential staining between the various epithelial populations was not obtained. RAP-5 also showed other tissue components such as plasma cells, histiocytes, fibroblasts, smooth muscle and vascular endothelium. CA19-9 antibody recognizes an epithelial surface carbohydrate antigen originally derived from a human colorectal carcinoma cell line: it did not stain normal colorectal mucosa or adenomatous polyps, but showed focal expression of variable strength in regenerative, dysplastic, and cancerous mucosa in ulcerative colitis, and in non-colitic colorectal carcinoma. Neither antibody was found to be a reliable marker of the evolution of malignant mucosal changes, although CA19-9 may be of limited use in confirming adenocarcinoma of gastrointestinal origin.

摘要

采用免疫组织化学技术，将两种单克隆抗体应用于经福尔马林固定、石蜡包埋的人良性、发育异常和恶性结直肠组织。RAP - 5抗体针对一种合成肽，该肽反映了原癌基因p21蛋白产物的氨基酸序列。尽管使用了几种不同的技术和抗体稀释度，但并未在不同上皮细胞群体之间获得差异染色。RAP - 5还显示出其他组织成分，如浆细胞、组织细胞、成纤维细胞、平滑肌和血管内皮细胞。CA19 - 9抗体识别一种最初源自人结肠癌细胞系的上皮表面碳水化合物抗原：它不染色正常结直肠黏膜或腺瘤性息肉，但在溃疡性结肠炎的再生、发育异常和癌性黏膜以及非结肠炎相关性结直肠癌中显示出不同强度的局灶性表达。尽管CA19 - 9在确认胃肠道起源的腺癌方面可能有有限的用途，但两种抗体均未被发现是恶性黏膜变化演变的可靠标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da0a/1140859/2495e258bce4/jclinpath00322-0037-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da0a/1140859/ccf29b13d6e9/jclinpath00322-0034-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da0a/1140859/524f06404d76/jclinpath00322-0035-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da0a/1140859/00b35eb63998/jclinpath00322-0036-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da0a/1140859/2495e258bce4/jclinpath00322-0037-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da0a/1140859/ccf29b13d6e9/jclinpath00322-0034-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da0a/1140859/524f06404d76/jclinpath00322-0035-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da0a/1140859/00b35eb63998/jclinpath00322-0036-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da0a/1140859/2495e258bce4/jclinpath00322-0037-a.jpg

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本文引用的文献

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Enzyme-labeled antibodies: preparation and application for the localization of antigens.酶标抗体：抗原定位的制备与应用
J Histochem Cytochem. 1966 Dec;14(12):929-31. doi: 10.1177/14.12.929.
2
DEMONSTRATION OF TUMOR-SPECIFIC ANTIGENS IN HUMAN COLONIC CARCINOMATA BY IMMUNOLOGICAL TOLERANCE AND ABSORPTION TECHNIQUES.通过免疫耐受和吸收技术证明人类结肠癌中的肿瘤特异性抗原
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Oncogenes in solid human tumours.人类实体瘤中的癌基因。
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A monoclonal antibody-defined antigen associated with gastrointestinal cancer is a ganglioside containing sialylated lacto-N-fucopentaose II.一种与胃肠道癌相关的单克隆抗体定义抗原是一种含有唾液酸化乳糖-N-岩藻五糖II的神经节苷脂。
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Pathology of colorectal adenomas: a colonoscopic survey.结直肠腺瘤的病理学：一项结肠镜检查调查。
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Immunohistochemical demonstration of carcinoembryonic antigen in normal, transitional and inflamed colonic mucosa.癌胚抗原在正常、移行和炎症性结肠黏膜中的免疫组织化学显示
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Radioimmunometric assay for a monoclonal antibody-defined tumor marker, CA 19-9.针对单克隆抗体定义的肿瘤标志物CA 19-9的放射免疫分析。
Clin Chem. 1983 Mar;29(3):549-52.
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Gastrointestinal cancer-associated antigen in immunoperoxidase assay.免疫过氧化物酶测定中的胃肠道癌相关抗原
Cancer Res. 1982 Nov;42(11):4820-3.
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Elevated expression of the human ras oncogene family in premalignant and malignant tumours of the colorectum.人类ras癌基因家族在结直肠癌癌前病变及恶性肿瘤中的表达升高。
Br J Cancer. 1984 Jun;49(6):681-8. doi: 10.1038/bjc.1984.108.
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Dysplasia in inflammatory bowel disease: standardized classification with provisional clinical applications.炎症性肠病中的发育异常：标准化分类及临时临床应用
Hum Pathol. 1983 Nov;14(11):931-68. doi: 10.1016/s0046-8177(83)80175-0.