Borena Bizunesh Mideksa, Dilgasa Lemma, Gebremedhin Endrias Zewdu, Sarba Edilu Jorga, Marami Lencho Megersa, Kelbesa Kebede Abdisa, Tadese Nega Desalegn
Department of Veterinary Sciences, School of Veterinary Medicine, Ambo University, P.O. Box 19, Ambo, Ethiopia.
Department of Veterinary Laboratory Technology, School of Veterinary Medicine, Ambo University, P.O. Box 19, Ambo, Ethiopia.
Vet Med Int. 2022 Apr 14;2022:5643478. doi: 10.1155/2022/5643478. eCollection 2022.
A cross-sectional study was conducted to estimate the prevalence and associated risk factors of species and assess the antibiogram of () isolated from milk and milk products from Holeta, Ambo, and Bako towns, Ethiopia. A total of 482 samples (384 milk, 35 cottage cheeses, 30 bulk tank milk, and 33 curdle milk) were collected using a systematic random sampling method and isolation and identification of species were done using standard microbiological techniques. An antimicrobial susceptibility test for was performed using the Kirby-Bauer disk diffusion technique. Descriptive statistics were used to summarize the prevalence of while the Chi-square test and logistic regression were used to determine the association between the prevalence of and the risk factors and the magnitude of association, respectively. The overall isolation rate of species from milk and milk products was 7.67% (37/482; 95% confidence interval (CI): 5.46, 10.42). The highest prevalence of species (15.15%; 95% CI: 5.11-31.90) was detected in bulk tank milk and the lowest prevalence of species (6.67%; 95% CI: 0.82-22.07) and (0.00; 95% CI: 0.00-1.15) was found in curdled milk. The other species isolated were 0.62% (3/482; 95% CI: 0.13-1.81), 1.04% (5/482; 95% CI: 0.33-2.40), 1.24%, (6/482; 95% CI: 0.45-2.68), and 2.49% (12/482; 95% CI: 5.46-10.42). Univariable logistic regression showed that study town, herd size, farm size, number of lactating cows, and management system were the factors significantly associated with the isolation of species at farm level, while the intensive management system was the independent predictor at cow level in the multivariable model (adjusted odds ratio = 3.38, =0.046). isolates showed the highest resistance against oxacillin (100%), amoxicillin (90.91%), and vancomycine (81.82%). showed a very high multidrug resistance (MDR) [81.82%]. In conclusion, the current study showed the widespread type of species MDR isolates in cow raw milk and milk products from Ambo, Holeta, and Bako towns, Oromia Regional State, Ethiopia.
开展了一项横断面研究，以估计[具体物种]的流行率及相关风险因素，并评估从埃塞俄比亚霍莱塔、安博和巴科镇的牛奶及奶制品中分离出的[具体物种]的抗菌谱。采用系统随机抽样方法共收集了482份样本（384份牛奶、35份农家奶酪、30份大容量冷藏罐牛奶和33份凝乳），并使用标准微生物技术对[具体物种]进行分离和鉴定。采用 Kirby-Bauer 纸片扩散技术对[具体物种]进行药敏试验。描述性统计用于总结[具体物种]的流行率，而卡方检验和逻辑回归分别用于确定[具体物种]的流行率与风险因素之间的关联以及关联强度。牛奶和奶制品中[具体物种]的总体分离率为7.67%（37/482；95%置信区间（CI）：5.46，10.42）。在大容量冷藏罐牛奶中检测到的[具体物种]流行率最高（15.15%；95%CI：5.11 - 31.90），而在凝乳中发现的[具体物种]流行率最低（6.67%；95%CI：0.82 - 22.07），[另一物种]的流行率为（0.00；95%CI：0.00 - 1.15）。分离出的其他物种有[物种名称1]0.62%（3/482；95%CI：0.13 - 1.81），[物种名称2]1.04%（5/482；95%CI：0.33 - 2.40），[物种名称3]1.24%（6/482；95%CI：0.45 - 2.68），以及[物种名称4]2.49%（12/482；95%CI：5.46 - 10.42）。单变量逻辑回归显示，研究城镇、畜群规模、农场规模、泌乳奶牛数量和管理制度是农场层面与[具体物种]分离显著相关的因素，而在多变量模型中，集约化管理制度是奶牛层面的独立预测因素（调整优势比 = 3.38，P = 0.046）。[具体物种]分离株对苯唑西林（100%）、阿莫西林（90.91%）和万古霉素（81.82%）表现出最高耐药性。[具体物种]表现出非常高的多重耐药性（MDR）[81.82%]。总之，当前研究表明，在埃塞俄比亚奥罗米亚州安博、霍莱塔和巴科镇的奶牛生鲜乳及奶制品中，[具体物种]多重耐药分离株广泛存在。
