Gebremedhin Endrias Zewdu, Soboka Gezahegn Tafesse, Borana Bizunesh Mideksa, Marami Lencho Megersa, Sarba Edilu Jorga, Tadese Nega Desalegn, Ambecha Hirut Abebe
Ambo University, College of Agriculture and Veterinary Sciences, Department of Veterinary Sciences, P.O. Box 19, Ambo, Ethiopia.
West Shewa Zone, Ambo District, Ambo, Ethiopia.
Int J Microbiol. 2021 Mar 23;2021:6626373. doi: 10.1155/2021/6626373. eCollection 2021.
has been recognized as a major cause of food-borne illness associated with the consumption of food of animal origin. The present cross-sectional study was conducted from December 2017 to May 2018 in Ambo and Holeta towns to assess the prevalence, risk factors, and antimicrobial susceptibility patterns of nontyphoidal isolates from raw beef samples from abattoirs, butchers, and restaurants in Ambo and Holeta towns, Oromia region, Ethiopia.
A total of 354 beef samples were collected from abattoirs, butchers, and restaurants. isolation and identification were carried out using standard bacteriological methods recommended by the International Organization for Standardization. Antimicrobial susceptibility testing was performed using the disk diffusion method. Besides, a structured questionnaire was used to collect sociodemographic data and potential risk factors for contamination of meat. Chi-square tests and logistic regression were used for data analyses.
Of the total 354 meat samples examined, 20 (5.7%, 95% confidence interval (CI): 3.5-8.6) were positive for . Two serotypes belonging to (11 isolates) and I:4,5,12: i:- (9 isolates) were identified. The detection rate in abattoirs, butchers, and restaurants was 4.2% (5/118), 8.5% (10/118), and 4.2% (5/118), respectively. The antimicrobial susceptibility test showed that 40%, 30%, and 20% of the isolates were resistant to azithromycin, amoxicillin, and ceftriaxone, respectively. The odds of isolation when meat handlers are illiterate were 7.8 times higher than those when they are educated to the level of secondary and above (=0.032). Similarly, the likelihood of isolation was 6.3 and 7.6 times higher among workers of butcher and restaurants, respectively, who had no training (=0.003) and no knowledge (=0.010) on food safety and hygiene.
The study showed widespread multidrug-resistant isolates in the study areas. Therefore, raw meat consumption and indiscriminate use of antimicrobial drugs should be discouraged. Provision of food safety education for meat handlers and further surveillance of antimicrobial-resistant isolates are suggested.
已被公认为是与食用动物源性食品相关的食源性疾病的主要病因。本横断面研究于2017年12月至2018年5月在安博和霍莱塔镇开展，旨在评估埃塞俄比亚奥罗米亚地区安博和霍莱塔镇屠宰场、肉店及餐厅生牛肉样本中非伤寒 分离株的流行情况、危险因素及抗菌药物敏感性模式。
从屠宰场、肉店及餐厅共采集了354份牛肉样本。采用国际标准化组织推荐的标准细菌学方法进行分离和鉴定。采用纸片扩散法进行抗菌药物敏感性试验。此外，使用结构化问卷收集社会人口统计学数据及肉类污染的潜在危险因素。采用卡方检验和逻辑回归进行数据分析。
在总共检测的354份肉类样本中，20份（5.7%，95%置信区间（CI）：3.5 - 8.6） 检测呈阳性。鉴定出属于 的两种血清型（11株分离株）和I:4,5,12:i:-（9株分离株）。屠宰场、肉店及餐厅的 检出率分别为4.2%（5/118）、8.5%（10/118）和4.2%（5/118）。抗菌药物敏感性试验表明，分别有40%、30%和20%的 分离株对阿奇霉素、阿莫西林和头孢曲松耐药。肉类处理人员为文盲时 分离的几率比受过中等及以上教育者高7.8倍（=0.032）。同样，在没有接受过食品安全和卫生培训（=0.003）以及没有相关知识（=0.010）的肉店和餐厅工作人员中， 分离的可能性分别高6.3倍和7.6倍。
该研究表明研究区域内存在广泛的多重耐药 分离株。因此，应劝阻食用生肉和滥用抗菌药物。建议为肉类处理人员提供食品安全教育，并进一步监测耐药分离株。
