Department of Biology, Cellular and Developmental Biology, Payame Noor University, Isfahan, Iran.
Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
Reprod Domest Anim. 2022 Aug;57(8):876-885. doi: 10.1111/rda.14131. Epub 2022 May 11.
Overproduction of reactive oxygen species (ROS) during sperm cryopreservation has a detrimental effect on sperm parameters. Therefore, the use of antioxidants in the sperm freezing extender can reduce ROS destructive effects. In this study, we investigated whether co-supplementation of melatonin and myo-inositol into the semen extender can improve the post-cryopreservation quality of goat spermatozoa. After the freeze-thawing process, sperm motility, viability, plasma membrane and acrosome intact morphology were improved in the combined myo-inositol and melatonin group compared to both individual and the control groups (p < .05). In addition, the mean of sperm ROS, DNA damage and lipid peroxidation were reduced in co-supplementation of myo-inositol and melatonin compared to their individual counterparts (p < .05). Therefore, the synergistic effects of myo-inositol and melatonin on the cryopreserved spermatozoa are highly likely mediated through the reduction in important factors involved in the sperm lipid peroxidation. Finally, we used the cryopreserved spermatozoa for in vitro production of embryos. Results showed that combined group of myo-inositol and melatonin improved the cleavage rate compared to both individual and control groups, although blastocyst rate was improved using both individual and combined groups. In conclusion, co-supplementation of melatonin and myo-inositol is a promising approach for the improvement of goat sperm cryopreservation.
在精子冷冻保存过程中，活性氧（ROS）的过度产生对精子参数有不利影响。因此，在精子冷冻保存液中使用抗氧化剂可以减少 ROS 的破坏作用。在这项研究中，我们研究了在精液冷冻保存液中共同补充褪黑素和肌醇是否可以改善山羊精子的冷冻后质量。与单独组和对照组相比，在冻融过程后，联合补充肌醇和褪黑素组的精子运动能力、活力、质膜和顶体完整形态都得到了改善（p<0.05）。此外，与单独补充相比，联合补充肌醇和褪黑素可以降低 ROS、DNA 损伤和脂质过氧化（p<0.05）。因此，肌醇和褪黑素对冷冻保存精子的协同作用很可能是通过降低与精子脂质过氧化相关的重要因素来介导的。最后，我们使用冷冻保存的精子进行体外胚胎生产。结果表明，与单独组和对照组相比，联合补充肌醇和褪黑素组的卵裂率得到了提高，尽管单独组和联合组都提高了囊胚率。总之，联合补充褪黑素和肌醇是提高山羊精子冷冻保存的一种有前途的方法。
