Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CiQUS), Departamento de Física de Partículas, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CiQUS), Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Nanoscale. 2022 May 16;14(18):6789-6801. doi: 10.1039/d1nr08305h.
The synthesis of nanosized metal-organic frameworks (NMOFs) is requisite for their application as injectable drug delivery systems (DDSs) and other biorelevant purposes. Herein, we have critically examined the role of different synthetic parameters leading to the production of UiO-66 crystals smaller than 100 nm. Of note, we demonstrate the co-modulator role conferred by halide ions, not only to produce NMOFs with precise morphology and size, but also to significantly improve the reaction yield. The resulting NMOFs are highly crystalline and exhibit sustained colloidal stability in different biologically relevant media. As a proof of concept, these NMOFs were loaded with Rhodamine 6G (R6G), which remained trapped in most common biologically relevant media. When incubated with living mammalian cells, the R6G-loaded NMOFs were efficiently internalized and did not impair cell viability even at relatively high doses.
纳米级金属有机骨架（NMOFs）的合成对于其作为可注射药物传递系统（DDS）和其他生物相关用途的应用是必要的。在此，我们批判性地研究了导致 UiO-66 晶体小于 100nm 的不同合成参数的作用。值得注意的是，我们证明了卤化物离子赋予的共调节剂作用，不仅可以生产具有精确形态和尺寸的 NMOFs，而且可以显著提高反应产率。得到的 NMOFs 是高度结晶的，并在不同的生物相关介质中表现出持续的胶体稳定性。作为概念验证，这些 NMOFs 被负载了 Rhodamine 6G（R6G），其在大多数常见的生物相关介质中仍然被捕获。当与活哺乳动物细胞孵育时，负载 R6G 的 NMOFs 被有效内化，并且即使在相对高剂量下也不会损害细胞活力。
