School of Agricultural Engineering and Food Science, Shandong University of Technology, 266 Xincun Xilu, Zibo, Shandong 255049, People's Republic of China.
Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, 266 Xincun Xilu, Zibo, Shandong 255049, People's Republic of China.
J Agric Food Chem. 2022 May 11;70(18):5477-5498. doi: 10.1021/acs.jafc.2c01547. Epub 2022 Apr 26.
Pathogenic bacteria are primarily kinds of detrimental agents that cause mankind illness via contaminated food with traits of multiple types, universality, and low content. In view of the detection demands for rapidity, aptamer recognition factors emerged as a substitution for antibodies, which are short single strands of nucleic acid selected via . They display certain superiorities over antibodies, such as preferable stability, liable modification, and cost-efficiency. Taking advantage of the situation, numerous aptamers against pathogenic bacteria have been successfully selected and applied, yet there are still restrictions on commercial availability. In this review, the strategies/approaches to key sections in pathogen aptamers SELEX and post-SELEX are summarized and sorted out. Recently, optical, electrochemical, and piezoelectric aptamer-based assays or sensors dedicated to pathogen detection have been critically reviewed. Ultimately, the existing challenges and future trends in this field are proposed to further promote development prospects.
病原微生物主要是指通过具有多种类型、普遍性和低含量特征的污染食物而导致人类疾病的有害物。鉴于对快速检测的需求，适体识别因子作为抗体的替代品出现，它是通过指数富集配体系统进化技术筛选出的短的单链核酸。与抗体相比，它们具有某些优势，如更好的稳定性、易于修饰和成本效益。利用这一情况，已经成功地筛选和应用了许多针对病原微生物的适体，但在商业可用性方面仍存在限制。在这篇综述中，总结和梳理了病原微生物适体 SELEX 及其后续 SELEX 中关键部分的策略/方法。最近，专门用于病原体检测的光学、电化学和压电适体基分析或传感器也得到了批判性的审查。最后，提出了该领域目前存在的挑战和未来的发展趋势，以进一步推动其发展前景。
