Opt Express. 2022 Apr 25;30(9):14709-14722. doi: 10.1364/OE.451919.
Airy light-sheet microscopy is rapidly gaining importance for imaging intact biological specimens because of the rapid speed, high resolution, and wide field nature of the imaging method. However, the depth of field (DOF) of the detection objective imposes limitations on the modulation transfer function (MTF) of the light sheet, which in turn affects the size of the field of view (FOV). Here we present an optimized phase modulation model, based on 'Airy-like' beam family, to stretch the curved lobes, which brings a wider FOV while maintaining high resolution. In addition, we further develop a planar 'Airy-like' light-sheet by two-photon excitation which can avoid the deconvolution process. We validated the new imaging method by performing a real-time monitoring of the dynamic process of cerebral hemorrhage in zebrafish larva. The proposed Airy-like beam-based light-sheet microscopy has great potential to be applied to the precise screening of cerebral hemorrhage-related drugs to help precision medicine in the future.
空气光片显微镜由于其成像速度快、分辨率高、视场宽，在对完整生物样本成像方面的重要性日益凸显。然而，检测物镜的景深（DOF）限制了光片的调制传递函数（MTF），从而影响了视场（FOV）的大小。在这里，我们提出了一种基于“类艾里光束族”的优化相位调制模型，该模型可以拉伸弯曲的波瓣，从而在保持高分辨率的同时获得更宽的 FOV。此外，我们还通过双光子激发进一步开发了一种平面“类艾里”光片，可避免去卷积过程。我们通过实时监测斑马鱼幼虫脑出血的动态过程验证了新的成像方法。基于类艾里光束的光片显微镜具有很大的潜力应用于脑出血相关药物的精确筛选，以帮助未来的精准医学。
