Academy of Medical Engineering and Translational Medicine, Tianjin University, Tianjin 300072, China.
School of Precision Instruments and Optoelectronics Engineering, Tianjin University, Tianjin 300072, China.
Sensors (Basel). 2023 Sep 6;23(18):7689. doi: 10.3390/s23187689.
(1) Objective: To explore the neurobiological effects of terahertz (THz) radiation on zebrafish larvae using calcium (Ca) imaging technology. (2) Methods: Zebrafish larvae at 7 days post fertilization (dpf) were exposed to THz radiation for 10 or 20 min; the frequency was 2.52 THz and the amplitude 50 mW/cm. The behavioral experiments, neural Ca imaging, and quantitative polymerase chain reaction (qPCR) of the dopamine-related genes were conducted following the irradiation. (3) Results: Compared with the control group, the behavioral experiments demonstrated that THz radiation significantly increased the distance travelled and speed of zebrafish larvae. In addition, the maximum acceleration and motion frequency were elevated in the 20 min radiation group. The neural Ca imaging results indicated a substantial increase in zebrafish neuronal activity. qPCR experiments revealed a significant upregulation of dopamine-related genes, such as , , and . (4) Conclusion: THz radiation (2.52 THz, 50 mW/cm, 20 min) upregulated dopamine-related genes and significantly enhanced neuronal excitability, and the neurobiological effect of THz radiation can be visualized using neural Ca imaging in vivo.
(1) 目的：利用钙成像技术探讨太赫兹（THz）辐射对斑马鱼幼鱼的神经生物学效应。(2) 方法：将受精后 7 天（dpf）的斑马鱼幼鱼暴露于 THz 辐射下 10 或 20 分钟；频率为 2.52 THz，振幅为 50 mW/cm。照射后进行行为实验、神经钙成像和多巴胺相关基因的定量聚合酶链反应（qPCR）。(3) 结果：与对照组相比，行为实验表明，THz 辐射显著增加了斑马鱼幼鱼的游动距离和速度。此外，20 分钟辐射组的最大加速度和运动频率也有所提高。神经钙成像结果表明斑马鱼神经元活动显著增加。qPCR 实验显示，多巴胺相关基因如 、 、 和 显著上调。(4) 结论：THz 辐射（2.52 THz，50 mW/cm，20 分钟）上调了多巴胺相关基因，并显著增强了神经元兴奋性，THz 辐射的神经生物学效应可以通过体内神经钙成像来可视化。
