McDougall J, Nazar R N
Nucleic Acids Res. 1987 Jan 12;15(1):161-79. doi: 10.1093/nar/15.1.161.
Comparative studies have been undertaken on the higher order structure of ribosomal 5S RNAs from diverse origins. Competitive reassociation studies show that 5S RNA from either a eukaryote or archaebacterium will form a stable ribonucleoprotein complex with the yeast ribosomal 5S RNA binding protein (YL3); in contrast, eubacterial RNAs will not compete in a similar fashion. Partial S1 ribonuclease digestion and ethylnitrosourea reactivity were used to probe the structural differences suggested by the reconstitution experiments. The results indicate a more compact higher order structure in eukaryotic 5S RNAs as compared to eubacteria and suggest that the archaebacterial 5S RNA contains features which are common to either group. The potential significance of these results with respect to a generalized model for the tertiary structure of the ribosomal 5S RNA and to the heterogeneity in the protein components of 5S RNA-protein complexes are discussed.
已经对来自不同来源的核糖体5S RNA的高级结构进行了比较研究。竞争性重配实验表明，真核生物或古细菌的5S RNA会与酵母核糖体5S RNA结合蛋白（YL3）形成稳定的核糖核蛋白复合物；相比之下，细菌RNA不会以类似方式竞争。利用部分S1核糖核酸酶消化和乙基亚硝基脲反应性来探究重组实验所表明的结构差异。结果表明，与细菌相比，真核生物5S RNA的高级结构更为紧凑，并且表明古细菌5S RNA含有两组共有的特征。讨论了这些结果对于核糖体5S RNA三级结构的通用模型以及5S RNA-蛋白质复合物蛋白质成分异质性的潜在意义。
