Küntzel H, Piechulla B, Hahn U
Nucleic Acids Res. 1983 Feb 11;11(3):893-900. doi: 10.1093/nar/11.3.893.
A consensus structure model of 5S rRNA presenting all conserved nucleotides in fixed positions has been deduced from the primary and secondary structure of 71 eubacterial, archaebacterial, eukaryotic cytosolic and organellar molecules. Phylogenetically related groups of molecules are characterized by nucleotide deletions in helices III, IV and V, and by potential base pair interactions in helix IV. The group-specific deletions are correlated with the early branching pattern of a dendrogram calculated from nucleotide substitution data: the first major division separates the group of eubacterial and organellar molecules from a second group containing the common ancestors of archaebacterial and eukaryotic/cytosolic molecules. The earliest diverging branch of the eubacterial/organellar group includes molecules from Thermus thermophilus, T. aquaticus, Rhodospirillum rubrum, Paracoccus denitrificans and wheat mitochondria.
根据71种真细菌、古细菌、真核细胞质和细胞器分子的一级和二级结构，推导了一种5S rRNA的共有结构模型，该模型呈现了所有固定位置的保守核苷酸。系统发育相关的分子组的特征在于螺旋III、IV和V中的核苷酸缺失，以及螺旋IV中的潜在碱基对相互作用。特定组的缺失与根据核苷酸替换数据计算的树状图的早期分支模式相关：第一个主要分支将真细菌和细胞器分子组与包含古细菌和真核/细胞质分子共同祖先的第二组分开。真细菌/细胞器组中最早分化的分支包括嗜热栖热菌、嗜热水生栖热菌、深红红螺菌、反硝化副球菌和小麦线粒体的分子。
