哺乳动物细胞中不可水解的磷酸酪氨酸类似物的遗传编码。
Genetic encoding of a nonhydrolyzable phosphotyrosine analog in mammalian cells.
作者信息
He Xinyuan, Ma Bin, Chen Yan, Guo Jiantao, Niu Wei
机构信息
Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, Nebraska, 68588, USA.
Department of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska, 68588, USA.
出版信息
Chem Commun (Camb). 2022 May 12;58(39):5897-5900. doi: 10.1039/d2cc01578a.
Protein tyrosine phosphorylation plays a critical role in signal transduction. We report the genetic incorporation of a phosphotyrosine (pTyr) analog, -carboxymethyl-L-phenylalanine (CMF), into proteins in mammalian cells. This nonhydrolyzable pTyr analog can facilitate biological studies by removing complications caused by the dynamic interconversion between the phosphorylated and non-phosphorylated isoforms of a protein.
蛋白质酪氨酸磷酸化在信号转导中起关键作用。我们报道了在哺乳动物细胞中，将磷酸酪氨酸（pTyr）类似物——羧甲基-L-苯丙氨酸（CMF）基因掺入蛋白质中。这种不可水解的pTyr类似物可以通过消除因蛋白质磷酸化和非磷酸化异构体之间的动态相互转化所引起的复杂性，来促进生物学研究。
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