Textile Materials Technology, Department of Textile Technology, Faculty of Textiles, Engineering and Business/The Swedish School of Textiles, University of Borås, 501 90, Borås, Sweden.
Sci Rep. 2022 Apr 26;12(1):6828. doi: 10.1038/s41598-022-10852-2.
Tyrosinase enzyme was digitally printed on plasma pretreated polyamide-6,6 fabric using several sustainable technologies. Ink containing carboxymethyl cellulose was found to be the most suitable viscosity modifier for this enzyme. Before and after being deposited on the fabric surface, the printed inks retained enzyme activity of 69% and 60%, respectively, compared to activity prior printing process. A good number of the printed enzyme was found to be strongly adsorbed on the fabric surface even after several rinsing cycles due to surface activation by plasma treatment. Rinsed out fabrics retained a maximum activity of 34% resulting from the well-adsorbed enzymes. The activity of tyrosinase on printed fabrics was more stable than ink solution for at least 60 days. Effects of pH, temperature and enzyme kinetics on ink solution and printed fabrics were assessed. Tyrosinase printed synthetic fabrics can be utilized for a range of applications from biosensing and wastewater treatment to cultural heritage works.
采用多种可持续技术，将酪氨酸酶酶制剂通过数码打印的方式印制在经过等离子体预处理的聚酰胺-6,6 纤维织物上。结果表明，含有羧甲基纤维素的墨水是最适合该酶的粘度调节剂。与打印前相比，打印后墨水在沉积于织物表面前后分别保留了 69%和 60%的酶活性。由于等离子体处理对表面的激活作用，大量的打印酶即使经过多次冲洗循环也能强烈地吸附在织物表面上。经过冲洗的织物由于吸附良好的酶而保留了最高达 34%的最大活性。打印织物上的酪氨酸酶的活性至少在 60 天内比墨水溶液更稳定。评估了 pH 值、温度和酶动力学对墨水溶液和打印织物的影响。用于打印的酪氨酸酶合成织物可用于从生物传感和废水处理到文化遗产工作等一系列应用。
