Department of Bioengineering, School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Department of Chemistry & Biotechnology, School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
PLoS One. 2022 Apr 27;17(4):e0266474. doi: 10.1371/journal.pone.0266474. eCollection 2022.
Respiratory infectious diseases pose a serious threat worldwide, and novel antiviral materials are highly demanded. Photocatalytic nanoparticles have been developed to inhibit indirect transmission of pathogens by acting as surface coating materials. During development of such antiviral materials, researchers use bacteriophages as model viruses due to their safety and experimental efficiency. Screening methods are used to identify potential antiviral materials, and better screening technologies will accelerate the discovery of antiviral treatments. In this study, we constructed a novel platform to evaluate antiviral activity of surface coating materials using the M13 bacteriophage and phagemid system derived from phage display technology. The evaluation results generated by this system for the two tested antiviral materials were comparable to those for the materials tested on the Qβ bacteriophage and influenza virus using traditional screening methods. The experimental system developed in this study provides rapid and effective screening and can be applied to the development of novel antiviral materials.
呼吸道传染病在全球范围内构成严重威胁，因此对抗病毒材料的需求很高。光催化纳米颗粒已被开发出来，用作表面涂层材料以抑制病原体的间接传播。在开发此类抗病毒材料时，由于噬菌体的安全性和实验效率，研究人员将其用作模型病毒。筛选方法用于鉴定潜在的抗病毒材料，更好的筛选技术将加速抗病毒治疗的发现。在这项研究中，我们构建了一个使用 M13 噬菌体和噬菌体展示技术衍生的噬菌粒系统来评估表面涂层材料抗病毒活性的新平台。该系统对两种测试的抗病毒材料的评估结果与传统筛选方法对 Qβ噬菌体和流感病毒测试的材料的评估结果相当。本研究中开发的实验系统提供了快速有效的筛选，可用于新型抗病毒材料的开发。
