Institut de Biologia Molecular de Barcelona, CSIC, Parc Científic de Barcelona, Baldiri Reixac, 10-12, 08028 Barcelona, Spain.
Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide/CSIC/JA, Carretera de Utrera, 41013 Sevilla, Spain.
Cell Rep. 2022 Apr 26;39(4):110734. doi: 10.1016/j.celrep.2022.110734.
Biological tubes are fundamental units of most metazoan organs. Their defective morphogenesis can cause malformations and pathologies. An integral component of biological tubes is the extracellular matrix, present apically (aECM) and basally (BM). Studies using the Drosophila tracheal system established an essential function for the aECM in tubulogenesis. Here, we demonstrate that the BM also plays a critical role in this process. We find that BM components are deposited in a spatial-temporal manner in the trachea. We show that laminins, core BM components, control size and shape of tracheal tubes and their topology within the embryo. At a cellular level, laminins control cell shape changes and distribution of the cortical cytoskeleton component α-spectrin. Finally, we report that the BM and aECM act independently-yet cooperatively-to control tube elongation and together to guarantee tissue integrity. Our results unravel key roles for the BM in shaping, positioning, and maintaining biological tubes.
生物管是大多数后生动物器官的基本单位。它们形态发生的缺陷会导致畸形和病变。生物管的一个组成部分是细胞外基质，分别存在于顶端（aECM）和基底（BM）。使用果蝇气管系统的研究确立了 aECM 在管状形成中的重要功能。在这里，我们证明了 BM 也在这个过程中起着关键作用。我们发现 BM 成分在气管中以时空方式沉积。我们表明，层粘连蛋白是 BM 的核心成分，控制着气管管腔的大小和形状及其在胚胎中的拓扑结构。在细胞水平上，层粘连蛋白控制着细胞形状的变化和皮质细胞骨架成分α- spectrin 的分布。最后，我们报告说，BM 和 aECM 独立但协同地作用以控制管腔的伸长，并共同保证组织的完整性。我们的结果揭示了 BM 在塑造、定位和维持生物管中的关键作用。
