Amadeite SAS, 56580, Bréhan, France.
Olmix, SALe Lintan, 56580, Bréhan, France.
BMC Vet Res. 2022 Apr 27;18(1):155. doi: 10.1186/s12917-022-03247-y.
Marek's disease (MD) is a highly contagious lymphoproliferative disease of chickens caused by an alphaherpesvirus, Marek's disease virus (MDV). MD is presently controlled by systematic vaccination of animals, which protects efficiently against the development of clinical disease. However, MDV vaccines do not prevent the multiplication and spread of MDV field strains and may favor the emergence of strains with increased virulence. Therefore, MDV persists to be a major problem for the poultry industry and the development of new alternative strategies to control MDV is needed. Seaweed extracts have previously been shown to exert immunomodulatory and antiviral activities, especially against herpesviruses. The objective of the present study was to explore the effect of Ulva armoricana extracts on MDV infection in vitro.
We could demonstrate that the ulvan extract as well as its vitamin-enriched formulation reduce the viral load by about 80% at 24 h post-infection in infected chicken fibroblasts at concentrations that are innocuous for the cells. We also observed a substantial decrease in MDV plaque size suggesting that ulvans impede MDV cell-to-cell spread in vitro. Moreover, we showed that ulvan extract could promote MDV reactivation in lymphoid cells.
Our data provide the first evidence that the use of the ulvan extract could be a good alternative to limit MDV infection in poultry.
马立克氏病（MD）是一种由α疱疹病毒——马立克氏病病毒（MDV）引起的鸡的高度传染性淋巴增生性疾病。MD 目前通过对动物进行系统疫苗接种来控制，这可以有效地防止临床疾病的发生。然而，MDV 疫苗不能阻止 MDV 田间株的繁殖和传播，并且可能有利于毒力增加的菌株的出现。因此，MDV 仍然是家禽业的一个主要问题，需要开发新的替代策略来控制 MDV。海藻提取物先前已被证明具有免疫调节和抗病毒活性，特别是针对疱疹病毒。本研究的目的是探讨 U. armoricana 提取物对 MDV 感染的体外影响。
我们可以证明，在感染鸡成纤维细胞后 24 小时，在对细胞无毒的浓度下，角叉菜聚糖提取物及其富含维生素的配方可将病毒载量降低约 80%。我们还观察到 MDV 蚀斑大小的显著减少，表明角叉聚糖可阻碍 MDV 在体外的细胞间传播。此外，我们表明角叉聚糖提取物可促进淋巴样细胞中 MDV 的重新激活。
我们的数据首次提供了证据，表明使用角叉聚糖提取物可能是限制家禽 MDV 感染的一种很好的替代方法。
