Xue Benchu, Wu Mei, Yue Shuangming, Hu Anhai, Li Xiang, Hong Qionghua, Wang Zhisheng, Wang Lizhi, Peng Quanhui, Xue Bai
Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, China.
Department of Bioengineering, Sichuan Water Conservancy College, Chengdu, China.
Front Microbiol. 2022 Apr 11;13:820509. doi: 10.3389/fmicb.2022.820509. eCollection 2022.
Physically effective neutral detergent fiber (peNDF) is a concept that accounts for the particle length of NDF in a feed, sustaining the normal chewing behavior and rumen fermentation of ruminants. This study aimed to elucidate the effects of dietary peNDF on growth performance and bacterial communities in the rumen of goats through a high-throughput sequencing technique. A total of 30 male Lezhi black goats were randomly assigned to five groups, corresponding to five diets with identical compositions and nutrient levels but with varying forage lengths (the peNDF. contents of the diets were 33.0, 29.9, 28.1, 26.5, and 24.8%, respectively). The whole trial lasted for 44 days. As results show, feed intake and average daily gain were highest when peNDF. content was 26.5%, in which the papilla length of the dorsal sac in rumen was the highest. Chao1 and ACE indexes were similar among the treatments, while Shannon and Simpson indexes of the peNDF. = 28.1% group were the highest ( < 0.05). As the level of dietary peNDF. decreased, the dominant phylum transitioned from Bacteroidetes to Firmicutes. The top three dominant genera of rumen bacteria were , , and . They all showed a quadratic correlation with dietary peNDF. level ( < 0.05). The relative abundance of was positively correlated, while that of was negatively correlated, with amino acid metabolism and energy metabolism ( < 0.01). In conclusion, dietary peNDF level influenced goat growth performance, rumen development, and rumen bacterial community structures, and a peNDF1.18 level between 26.5 and 28.1% was considered optimal for goat diet.
物理有效中性洗涤纤维（peNDF）是一个考虑饲料中NDF颗粒长度的概念，它维持反刍动物的正常咀嚼行为和瘤胃发酵。本研究旨在通过高通量测序技术阐明日粮peNDF对山羊生长性能和瘤胃细菌群落的影响。总共30只乐至黑山羊被随机分为五组，分别对应五种组成和营养水平相同但牧草长度不同的日粮（日粮的peNDF含量分别为33.0%、29.9%、28.1%、26.5%和24.8%）。整个试验持续44天。结果表明，当peNDF含量为26.5%时，采食量和平均日增重最高，此时瘤胃背囊乳头长度也最高。各处理间Chao1和ACE指数相似，而peNDF = 28.1%组的Shannon和Simpson指数最高（P<0.05）。随着日粮peNDF水平的降低，优势菌门从拟杆菌门转变为厚壁菌门。瘤胃细菌的前三大优势属为……。它们均与日粮peNDF水平呈二次相关（P<0.05）。……的相对丰度与氨基酸代谢和能量代谢呈正相关，而……的相对丰度与氨基酸代谢和能量代谢呈负相关（P<0.01）。总之，日粮peNDF水平影响山羊生长性能、瘤胃发育和瘤胃细菌群落结构，26.5%至28.1%的peNDF水平被认为是山羊日粮的最佳水平。
