Sousa Filho Luis Fernando, Barbosa Santos Marta Maria, Menezes Paula Dos Passos, Lima Bruno Dos Santos, de Souza Araújo Adriano Antunes, de Oliveira Evaleide Diniz
Department of Physiotherapy, Federal University of Sergipe Av Marechal Rondon s/n 49000-100 São Cristovão Sergipe Brazil
Department of Pharmacy, Federal University of Sergipe Av Marechal Rondon s/n 49000-100 São Cristovão Sergipe Brazil.
RSC Adv. 2021 Aug 17;11(45):27837-27844. doi: 10.1039/d1ra04708f. eCollection 2021 Aug 16.
A gel containing the inclusion complex of quercetin and β-cyclodextrin was developed in order to verify its effects, isolated or using phonophoresis, on oxidative biomarkers after skeletal muscle injury. 30 male rats were divided into one of five groups: Control (CTRL), Muscle Injury (MI), Therapeutic Pulsed Ultrasound (TPU), Therapeutic Pulsed Ultrasound plus Quercetin (TPU plus gel-QUE) or Quercetin gel (QUE). Quercetin gel was complexed with β-Cyclodextrin (β-CD) using chromatography (HPLC). TPU and quercetin application occurred with 2, 12, 24, 48, 72, 96 hours intervals after injury. Gastrocnemius muscle was injured by mechanical trauma. Lipid peroxidation, superoxide dismutase activity, and catalase activity were assessed. The inclusion complex exhibited adequate entrapment efficiency, relative density and pH. The viscosity of the complex showed a non-Newtonian pseudoplastic behavior. Quercetin/β-cyclodextrin gel reduced lipid peroxidation, superoxide dismutase activity and catalase activity compared to muscle injury group. Similarly, phonophoresis and TPU also reduced the levels of these oxidative biomarkers. In conclusion, quercetin/β-cyclodextrin transdermal gel reduces oxidative stress biomarkers after skeletal muscle injury irrespective of using phonophoresis.
为了验证槲皮素与β - 环糊精包合物凝胶单独使用或经离子导入后对骨骼肌损伤后氧化生物标志物的影响，制备了该凝胶。30只雄性大鼠被分为五组之一：对照组（CTRL）、肌肉损伤组（MI）、治疗性脉冲超声组（TPU）、治疗性脉冲超声加槲皮素组（TPU加凝胶 - QUE）或槲皮素凝胶组（QUE）。使用色谱法（高效液相色谱法，HPLC）将槲皮素凝胶与β - 环糊精（β - CD）络合。在损伤后2、12、24、48、72、96小时的间隔时间进行TPU和槲皮素的应用。通过机械创伤损伤腓肠肌。评估脂质过氧化、超氧化物歧化酶活性和过氧化氢酶活性。包合物表现出足够的包封率、相对密度和pH值。该络合物的粘度表现出非牛顿假塑性行为。与肌肉损伤组相比，槲皮素/β - 环糊精凝胶降低了脂质过氧化、超氧化物歧化酶活性和过氧化氢酶活性。同样，离子导入和TPU也降低了这些氧化生物标志物的水平。总之，无论是否使用离子导入，槲皮素/β - 环糊精透皮凝胶均可降低骨骼肌损伤后的氧化应激生物标志物。
