College of Plant Protection, China Agricultural University, Beijing 100193, China.
College of Plant Protection, Shandong Agricultural University, Tai'an 271018, China.
J Agric Food Chem. 2022 May 11;70(18):5646-5657. doi: 10.1021/acs.jafc.2c00942. Epub 2022 Apr 28.
, the main agent of head blight (FHB), can cause serious yield loss and secrete mycotoxins to contaminate grain. Here, the biological activity of -2-hexenal (T2H) against was determined and its mode of action (MOA) was investigated. Furthermore, surface plasmon resonance with liquid chromatography-tandem mass spectrometry (SPR-LC-MS/MS), bioinformatic analysis, and gene knockout technique were combined to identify the binding proteins of T2H in cells. T2H exhibited satisfactory inhibitory activity against . Good lipophilicity greatly enhanced the affinity of T2H to mycelia and further caused membrane damage. The (thioredoxin reductase) gene negatively regulates the sensitivity of to T2H by reducing the generation of reactive oxygen species (ROS) induced by T2H. Two mutant strains with (structure-specific endonuclease subunit) and (coatomer subunit β) genes knockout showed decreased sensitivity to T2H, suggesting that these two genes may be involved in the antimicrobial activity of T2H. Taken together, T2H can inhibit growth by multiple MOAs and can be used as a biofumigant to control the occurrence of FHB in the field.
, 是小麦赤霉病（FHB）的主要侵染源，不仅会导致严重的产量损失，还会分泌真菌毒素污染粮食。本研究测定了 -2-己烯醛（T2H）对 的生物活性，并对其作用模式（MOA）进行了研究。此外，还结合表面等离子体共振与液相色谱-串联质谱（SPR-LC-MS/MS）、生物信息学分析和基因敲除技术，鉴定了 T2H 在 细胞中的结合蛋白。T2H 对 表现出良好的抑制活性。良好的亲脂性大大提高了 T2H 与 菌丝体的亲和力，并进一步导致了细胞膜损伤。 （硫氧还蛋白还原酶）基因通过降低 T2H 诱导的活性氧（ROS）的产生，负调控 对 T2H 的敏感性。两个缺失 （结构特异性内切酶亚基）和 （衣壳亚基β）基因的突变菌株对 T2H 的敏感性降低，表明这两个基因可能参与了 T2H 的抗菌活性。综上所述，T2H 可以通过多种 MOAs 抑制 的生长，可作为一种生物熏蒸剂，用于田间防控赤霉病的发生。
