Asa S L, Kovacs K, Vale W, Petrusz P, Vecsei P
Am J Clin Pathol. 1987 Mar;87(3):327-33. doi: 10.1093/ajcp/87.3.327.
The authors investigated formalin-fixed, paraffin-embedded human tissues for the presence of corticotrophin-releasing hormone (CRH) using the avidin-biotin-peroxidase technic. Immunopositivity was demonstrated in nontumorous hypothalami, 1 of 8 hypothalamic gangliocytomas, 3 of 9 bronchial endocrine tumors, 1 of 30 small cell lung carcinomas, 1 of 8 ileal endocrine neoplasms, 2 of 20 pancreatic endocrine tumors, 2 of 10 medullary thyroid carcinomas, and 1 of 3 small cell prostate carcinomas. Of the tumors containing immunoreactivity, most were associated with Cushing's syndrome; the ileal and thyroid tumors were not. Adrenocorticotrophic hormone was immunohistochemically localized in two bronchial and one pancreatic tumor, which contained CRH-like immunoreactivity. No CRH was detected in nontumorous extrahypothalamic tissues from which the CRH-containing tumors derived, two mediastinal endocrine carcinomas, six endocrine tumors of the stomach/duodenum/appendix, eight pheochromocytomas, one Merkel cell tumor, and 32 squamous and adenocarcinomas of the lung/gut. CRH-like immunoreactivity may be found in tumors composed of peptide-hormone-producing endocrine cells; hypersecretion of CRH by those neoplasms may be significant in the development of Cushing's syndrome.
作者使用抗生物素蛋白-生物素-过氧化物酶技术，研究了福尔马林固定、石蜡包埋的人体组织中促肾上腺皮质激素释放激素（CRH）的存在情况。在非肿瘤性下丘脑、8例下丘脑神经节细胞瘤中的1例、9例支气管内分泌肿瘤中的3例、30例小细胞肺癌中的1例、8例回肠内分泌肿瘤中的1例、20例胰腺内分泌肿瘤中的2例、10例甲状腺髓样癌中的2例以及3例小细胞前列腺癌中的1例中均显示出免疫阳性。在含有免疫反应性的肿瘤中，大多数与库欣综合征相关；回肠和甲状腺肿瘤则不然。促肾上腺皮质激素通过免疫组织化学定位在2例支气管肿瘤和1例胰腺肿瘤中，这些肿瘤含有CRH样免疫反应性。在源自含CRH肿瘤的非肿瘤性下丘脑外组织、2例纵隔内分泌癌、6例胃/十二指肠/阑尾内分泌肿瘤、8例嗜铬细胞瘤、1例默克尔细胞瘤以及32例肺/肠道鳞状细胞癌和腺癌中未检测到CRH。CRH样免疫反应性可能存在于由产生肽激素的内分泌细胞组成的肿瘤中；这些肿瘤分泌过多的CRH可能在库欣综合征的发生中起重要作用。
