Asa S L, Kovacs K, Thorner M O, Leong D A, Rivier J, Vale W
J Clin Endocrinol Metab. 1985 Mar;60(3):423-7. doi: 10.1210/jcem-60-3-423.
Two forms of GH-releasing factor (GHRH), which play a role in the regulation of GH secretion, have been isolated from pancreatic endocrine tumors in two patients with acromegaly. We examined formalin-fixed, paraffin-embedded human tissues from autopsies and surgical specimens for the presence of human pancreatic GHRH-40 using the avidin-biotin-peroxidase complex technique to assess the prevalence of tumors containing GHRH, to define their primary sites and cellular derivations, and to correlate clinical and pathological features. Immunopositivity was demonstrated in 4 of 24 pancreatic endocrine tumors, 1 of 5 bronchial and 2 of 15 gut carcinoids, 1 of 2 thymic carcinoids, 2 of 20 medullary carcinomas of the thyroid, 1 of 12 pheochromocytomas, and 5 of 20 small cell carcinomas of the lung. Of the GHRH-containing tumors, only 2 of the pancreatic endocrine tumors and the bronchial carcinoid were associated with acromegaly. No GHRH was found in 35 tumors derived from cells that are not known to produce peptide hormones. Immunoreactivity was not detected in the nontumorous tissues from which GHRH-containing tumors were derived. It can be concluded that GHRH may be found in a variety of tumors arising from and composed of peptide hormone-producing endocrine cells. The significance of immunoreactive GHRH detected in tumors unassociated with clinical evidence of acromegaly remains to be established.
从两名肢端肥大症患者的胰腺内分泌肿瘤中分离出了两种生长激素释放因子（GHRH），它们在生长激素分泌的调节中发挥作用。我们采用抗生物素蛋白-生物素-过氧化物酶复合物技术，检查了尸检和手术标本中经福尔马林固定、石蜡包埋的人体组织，以检测人胰腺GHRH-40的存在情况，从而评估含GHRH肿瘤的患病率，确定其原发部位和细胞来源，并将临床和病理特征相互关联。在24例胰腺内分泌肿瘤中有4例呈免疫阳性，5例支气管类癌中有1例、15例肠道类癌中有2例、2例胸腺类癌中有1例、20例甲状腺髓样癌中有2例、12例嗜铬细胞瘤中有1例、20例肺小细胞癌中有5例呈免疫阳性。在含GHRH的肿瘤中，只有2例胰腺内分泌肿瘤和支气管类癌与肢端肥大症有关。在35例源自未知产生肽类激素细胞的肿瘤中未发现GHRH。在含GHRH肿瘤所源自的非肿瘤组织中未检测到免疫反应性。可以得出结论，GHRH可能存在于源自肽类激素产生内分泌细胞并由其组成的多种肿瘤中。在与肢端肥大症临床证据无关的肿瘤中检测到的免疫反应性GHRH的意义尚待确定。
